1 affinity chromatography 1 chromatography 1 Copurification 7 Purification 9 chromatography 9 copurify 9 FLAG 9 immunoaffinity 9 immunoaffinity chromatography 9 purification 10 chromatography 10 FLAG 10 immunoaffinity 10 immunoaffinity chromatography 11 purification 12 pulldown 14 pulled down 15 TAP 16 purification 17 Chromatography 17 depletion 20 co-purified 27 Copurified 29 coexpression 29 copurified 29 immunoblots 31 antibody 31 anti-Flag 31 anti-HA 31 Flag 31 MALDI-TOF 31 mass spectrometric analysis 32 Immunoblotting 32 Immunoblotting analysis 35 in vitro 37 immunoprecipitations 37 purification 39 chromatography 41 purification 41 SDS-PAGE 41 SDS-PAGE analysis 42 antibodies 43 Gel filtration 47 MALDI-TOF 48 Western blot 50 elution 50 Fractionation 55 immunoprecipitation 55 immunoprecipitation experiments 57 coimmunoprecipitates 58 Antibodies 58 Antibodies raised against 58 immunoblot 58 immunoblot analysis 58 immunoprecipitate 58 precipitate 59 co-precipitated 59 co-purified 59 reciprocal experiment 60 immunoprecipitates 60 in vivo 62 antibodies 62 precipitate 65 Co-immunoprecipitation 67 immunoprecipitates 68 immunoprecipitates 70 coimmunoprecipitation 71 HA-tagged 71 immunoblotting 72 precipitates 73 antibodies 76 immunoprecipitates 77 nocodazole 80 coimmunoprecipitates 80 fusion protein 81 antibodies 81 coimmunoprecipitate 82 antibodies 84 antibody 85 antibody 94 Western blot 94 Western blot analysis 95 antibodies 95 coimmunoprecipitate 96 coprecipitated 97 co-precipitated 98 antibody 98 monoclonal antibody 99 in vivo 101 antibody 114 antibody 115 antibody 115 Western blot 115 Western blot analysis 116 Coimmunoprecipitation 117 antiserum 117 immunoprecipitates 118 coimmunoprecipitates 122 antibodies 123 coprecipitation 124 coprecipitates 124 reciprocal experiment 126 coimmunoprecipitation 127 immunoprecipitates 128 antibody 128 coprecipitate 129 antibodies 129 antibodies against 129 coimmunoprecipitate 131 coprecipitated 132 coprecipitated 133 immunoprecipitation 134 antibodies 134 antibody 134 coprecipitated 136 comigrated 136 immunoprecipitates 137 immunoprecipitates 143 antibodies 143 coprecipitated 143 immunoprecipitation 144 antibody 144 antiserum 144 Immunoprecipitation 144 western blots 145 fusion proteins 145 pull-down 145 pull-down assay 146 antiserum 146 immunoblotting 146 immunoprecipitation 147 immunoprecipitates 148 antibodies 148 antibodies against 148 coimmunoprecipitation 148 coimmunoprecipitation experiments 149 antibody 149 immunoprecipitates 149 immunoprecipitation 149 immunoprecipitation experiments 151 coimmunoprecipitation 152 coimmunoprecipitation 152 coimmunoprecipitation experiments 154 antibodies 154 coprecipitated 155 antibodies 155 western blotting 156 co-precipitated 156 MALDI 156 mass spectrometry 157 antibody 157 coprecipitates 157 Immunoprecipitation 158 antibody 158 fusion protein 158 GFP 161 antibody 161 co-immunoprecipitation 161 immunoprecipitation 161 monoclonal antibody 161 Western blot 161 Western blot analysis 162 antibody 162 coprecipitated 162 reciprocal experiment 163 immunoprecipitates 163 immunoprecipitations 165 coprecipitated 166 antibody 166 co-immunoprecipitation 166 co-precipitated 166 HA-tagged 166 In vivo 167 co-precipitated 167 GFP 168 antibodies 168 co-immunoprecipitate 168 reciprocal experiments 169 antibodies 169 co-immunoprecipitation 169 western blot 170 co-precipitated 171 Co-immunoprecipitation 172 antibody 173 antibody 173 coprecipitated 174 antibody 174 immunoprecipitation 175 Immunoprecipitation 176 antibody 176 coimmunoprecipitation 176 immunoblotting 176 in vitro 176 in vivo 179 antibody 179 anti-HA 179 HA-tagged 180 antibody 180 anti-HA 180 HA-tagged 181 antibody 182 Co-immunoprecipitation 182 Co-immunoprecipitation experiments 182 co-precipitated 183 co-purification 183 immunoblotting 183 immunoprecipitates 183 in vivo 184 fractionation 184 immunoprecipitation 185 immunoblotting 185 immunoblotting experiment 185 immunoprecipitation 186 Co-immunoprecipitation 188 in vitro 189 antibodies 189 immobilized 189 Western blotting 190 co-immunoprecipitation 190 In vitro 191 immunoprecipitation 191 immunoprecipitation assays 192 antibody 194 HA-tagged 198 antibodies 198 IP experiments 200 antibody 201 antibodies 201 immunoprecipitate 202 antibodies 206 immunoprecipitates 206 Western blots 207 immunoprecipitates 207 Western blots 208 antibodies 208 immunoprecipitations 212 Coimmunoprecipitation 213 in vivo 214 antibodies 214 immunoprecipitates 216 antibody 216 immunoprecipitates 216 western blotting 218 antibody 220 antibody 221 coimmunoprecipitation 221 coimmunoprecipitation assays 222 immunoprecipitate 223 immunoprecipitate 224 antibody 224 immunoprecipitate 225 immunoprecipitate 226 antibody 226 immunoblotting 228 coprecipitated 228 in vitro 230 antibody 232 coprecipitated 233 coprecipitated 235 Coimmunoprecipitation 236 antibody 236 immunoprecipitate 238 Immunoblot 238 Immunoblot analysis 238 precipitates 239 immunoblot 239 immunoblot analysis 239 in vitro 239 precipitates 240 Immunoblot 240 Immunoblot analysis 240 in vitro 240 precipitates 242 antibodies 243 Western blotting 244 Western blotting 246 antibodies 246 immunoprecipitate 248 antibody 248 pulled down 250 antibody 251 coprecipitated 252 in vivo 253 pull down 254 antibodies 254 antibodies against 254 Immunoblot 254 Immunoblot analysis 255 co-precipitate 255 Western blot 255 Western blot analysis 256 co-precipitate 256 Western blot 256 Western blot analysis 257 antibody 257 immunoprecipitation 257 in vivo 258 coprecipitated 259 coimmunoprecipitation 259 in vivo 260 co-immunoprecipitates 261 antibodies 262 coimmunoprecipitation 262 coimmunoprecipitation experiments 262 mass spectrometry 264 immunoblotting 265 antiserum 266 immunoblotting 267 coimmunoprecipitation 267 In vitro 268 co-immunoprecipitates 269 antibody 269 binding analysis 269 immunoprecipitation 269 Immunoprecipitations 270 antibody 270 binding analysis 270 fluorescence 270 GFP 270 green fluorescence 270 green fluorescence protein 270 immunoprecipitation 270 Immunoprecipitations 273 co-immunoprecipitates 274 copurified 274 Immunoprecipitation 274 Western blot 274 Western blot analysis 276 Coimmunoprecipitations 277 antibody 277 coprecipitated 278 antibody 279 Immunoblot 279 Immunoblot analysis 280 immunoprecipitate 280 Western blot 280 Western blot analysis 281 immunopurified 281 Western blotting 282 antibody 282 immunopurified 283 immunopurified 292 Coprecipitation 293 coprecipitated 294 IP assay 295 antibody 295 IP assay 296 immunoprecipitates 297 immunoprecipitates 298 antibody 299 antibodies 299 immunoprecipitation 300 immunoprecipitation 301 co-immunoprecipitate 302 in vivo 303 antiserum 303 co-precipitated 303 immunoprecipitation 305 antibodies 305 co-immunoprecipitation 309 co-precipitated 311 co-precipitated 311 FLAG-tagged 311 GST 311 immunoprecipitation 312 Western blotting 313 antibodies 313 immunoprecipitating 314 co-precipitated 314 immunoprecipitation 315 autoradiography 315 immunoprecipitation 315 immunoprecipitation experiments 315 SDS-PAGE 316 antibody 316 coimmunoprecipitation 316 Immunoblot 316 Immunoblot analysis 317 antibody 317 immunoprecipitation 317 immunoprecipitation assays 318 antibody 318 copurified 318 immunoblot 318 immunoblot assay 318 immunoprecipitates 318 reciprocal assay 319 antibodies 319 immunopurifications 319 western-blot 320 co-precipitation 323 co-immunoprecipitation 324 immunoprecipitation 325 Immunoprecipitation 326 antibody 326 Western blot 327 immunoprecipitation 328 antibody 328 immunoprecipitation 328 Western blot 329 anti-Flag 330 immunoprecipitation 331 immunoprecipitation 332 anti-Flag 332 immunoprecipitation 333 immunoprecipitation 334 antibodies 334 immunoprecipitation 336 antibodies 336 pulled down 337 antibody 337 Coimmunoprecipitating 337 monoclonal antibody 337 reciprocal experiments 337 Western blots 338 antibody 338 Co-precipitated 338 Western blotting 339 Antibody 339 coprecipitated 340 coprecipitate 341 coprecipitated 342 coimmunoprecipitate 343 coprecipitation 344 coprecipitation 345 antibodies 345 coprecipitated 346 in vivo 347 antibodies 347 antibodies against 347 coprecipitated 347 coprecipitation 348 antibody 349 in vivo 351 antibody 351 immunoprecipitating 352 antibody 352 co-immunoprecipitation 352 co-immunoprecipitation assay 352 co-precipitated 352 monoclonal antibody 354 antibody 354 precipitate 354 Western blotting 355 co-immunoprecipitate 363 antibodies 363 pull down 364 coimmunoprecipitate 367 antibody 368 antibody 368 database search 368 fingerprinting 368 Peptide mass fingerprinting 369 antibodies 369 antibody 369 immunoprecipitates 369 Western blotting 370 antibodies 370 antibody 370 immunoprecipitates 370 Western blotting 372 antibody 373 in vivo 374 antibody 374 coprecipitate 376 Co-immunoprecipitation 377 antibody 378 coimmunoprecipitates 381 antiserum 381 HA-tagged 383 antibodies 385 co-immunoprecipitation 385 reciprocal co-immunoprecipitation 385 Western blotting 386 co-immunoprecipitation 386 reciprocal co-immunoprecipitation 386 Western blotting 392 antibody 392 immunoprecipitation 392 in vivo 395 in vivo 397 co-immunoprecipitates 398 antibodies 398 antibody 398 coimmunoprecipitation 399 in vitro 399 in vivo 400 co-precipitate 401 co-precipitate 402 co-precipitate 403 reporter genes 404 immunoprecipitation 404 Western blot 405 immunoblotting 405 immunoprecipitation 405 Western blot 406 co-immunoprecipitation 406 reciprocal co-immunoprecipitation 407 immunoblotting 407 Immunoprecipitations 408 co-immunoprecipitations 408 Reciprocal co-immunoprecipitations 409 antibody 409 co-immunoprecipitation 410 precipitates 415 antibody 415 in vitro 417 Western blotting 419 in vitro 419 in vitro system 420 co-immunoprecipitation 420 co-immunoprecipitation analysis 420 in vitro 421 cosedimentation 422 in vivo 423 antibody 425 in vivo 427 Co-immunoprecipitation 427 Co-immunoprecipitation assay 428 co-precipitate 429 co-precipitated 431 immunoprecipitation 432 antibodies 432 anti-FLAG 432 FLAG-tagged 432 immunoprecipitation 433 antibodies 433 immunoblot 433 immunoblot analysis 433 Western blot 433 Western blot analysis 434 FLAG-tagged 435 antibodies 435 anti-FLAG 435 anti-GFP 435 immunoblot 435 immunoblot analysis 438 immunoprecipitation 438 pulled down 439 coprecipitate 439 immunoprecipitation 439 in vivo 441 Co-immunoprecipitation 441 Co-immunoprecipitation experiments 442 antibody 443 in vitro 443 in vivo 447 immunoprecipitate 448 Pull down 449 co-precipitated 449 immunoprecipitates 449 Western analysis 450 Western blotting 451 antibodies 451 immunoprecipitation 452 antibody 452 co-precipitated 452 Immunoprecipitations 452 Western blot 452 Western blot analysis 453 Immunoprecipitates 453 immunoprecipitation 453 immunoprecipitation experiment 453 immunoprecipitation experiments 454 pulled down 456 antibody 456 in vivo 457 co-immunoprecipitation 457 Western blot 457 Western blot analysis 458 immunoprecipitation 458 Western blot 459 immunoprecipitation 459 Western blot 460 antibodies 460 coimmunoprecipitation 461 antibodies 461 coimmunoprecipitates 461 immunoblotting 461 in vivo 462 coprecipitated 463 co-precipitated 464 co-precipitated 465 co-precipitated 466 antibody 466 precipitate 467 Immunoprecipitation 470 Co-immunoprecipitation 472 antibody 473 coimmunoprecipitations 475 co-precipitated 475 Immunoprecipitation 476 antibody 476 pull down 479 immunoprecipitates 480 immunoprecipitates 481 immunoprecipitates 483 antibody 483 anti-HA 483 HA-tagged 483 immunoprecipitation 483 Western blot 483 Western blot analysis 485 immunoprecipitates 486 co-immunoprecipitates 487 2-D gels 487 Immunopurified 488 antibodies 490 antibody 490 immunoprecipitates 492 co-precipitates 494 co-precipitate 496 antibodies 496 antibody 497 antibodies 497 antibodies against 497 antibody 497 immunoprecipitation 497 immunopurified 498 co-purified 498 immunoprecipitation 498 immunopurification 499 immunopurified 499 mock-treated 501 antibody 501 coimmunoprecipitation 501 colocalization 503 antibody 504 antibodies 504 Coimmunoprecipitation 504 Coimmunoprecipitation assays 508 antibody 509 co-immunoprecipitation 509 co-immunoprecipitation experiments 510 coimmunoprecipitation 510 coimmunoprecipitation experiments 513 antibody 515 antibodies 515 anti-HA 515 Coexpression 515 coimmunoprecipitation 516 antibodies 516 coimmunoprecipitation 516 Immunoprecipitation 517 coimmunoprecipitation 517 coimmunoprecipitation experiments 523 antibody 523 coprecipitation 523 mass spectrometry 526 antibody 526 co-precipitation 526 Immunoprecipitation 526 Immunoprecipitation assays 527 Western blotting 528 mass spectrometry 529 coprecipitated 529 in vivo 530 immunoprecipitation 530 immunoprecipitation assay 535 cotransfection 535 cotransfection assay 536 cotransfection 536 cotransfection assay 537 cotransfection 537 cotransfection assay 538 antibodies 538 coimmunoprecipitation 538 coimmunoprecipitation assay 538 reciprocal assay 539 antibodies 539 coimmunoprecipitation 539 coimmunoprecipitation assay 539 reciprocal assay 540 coimmunoprecipitation 540 coimmunoprecipitation assay 542 coprecipitated 543 co-precipitated 546 antibody 546 coprecipitated 546 immunoprecipitation 548 antibodies 548 Coimmunoprecipitations 549 coimmunoprecipitation 549 Pull-down 550 coimmunoprecipitates 552 antibodies 552 anti FLAG 552 co-immunoprecipitation 552 FLAG 552 immunoprecipitations 554 Immunoprecipitation 554 Immunoprecipitation experiments 556 precipitates 557 co-precipitated 557 immunoprecipitation 558 co-precipitated 559 co-precipitated 561 antibody 561 Immunoprecipitation 562 antibody 562 Immunoprecipitation 562 pulled down 563 immunoprecipitating 565 antibody 565 coprecipitated 565 precipitates 566 antibody 566 Immunodepletion 566 Immunodepletion experiment 566 immunoprecipitating 566 immunoprecipitation 566 western blotting 568 coprecipitation 568 coprecipitation experiments 569 coprecipitation 569 coprecipitation experiments 570 coimmunoprecipitation 570 coimmunoprecipitation experiments 573 antibody 576 mass spectrometry 576 purifications 577 in vivo 578 in vivo 583 coimmunoprecipitation 583 coimmunoprecipitation assay 585 immunoblotting 586 Immunoblot 586 Immunoblot experiments 587 antibodies 588 antibodies 588 co-precipitated 589 antibodies 589 co-precipitated 590 Western blots 591 Co-IP 599 HA-tagged 600 HA-tagged 602 coimmunoprecipitation 603 coprecipitated 605 antibody 605 coimmunoprecipitate 606 antibody 606 coimmunoprecipitate 607 GFP 609 GFP 610 coimmunoprecipitation 611 antibodies 611 immunoprecipitate 613 antibodies 613 anti-HA 613 coimmunoprecipitation 613 immunoblotting 616 coimmunoprecipitation 617 antibody 617 co-purified 617 immunoblotting 617 immunoprecipitation 618 antibodies 618 antibodies against 618 antibody 618 coimmunoprecipitation 618 coimmunoprecipitation experiment 618 immunoprecipitation 619 antibodies 619 antibodies against 619 antibody 619 coimmunoprecipitation 619 coimmunoprecipitation experiment 619 fusion protein 619 immunoprecipitates 619 immunoprecipitation 632 coimmunoprecipitation 632 mass spectrometry 632 mass spectrometry analysis 638 antibody 639 antibodies 640 antibody 640 coprecipitated 641 antibody 641 monoclonal antibody 642 anti-HA 642 antiserum 645 in vivo 648 precipitates 649 immunoprecipitates 653 overlay experiments 654 antibody 655 antibody 658 antibody 658 FLAG-tagged 658 immunoprecipitate 660 antibody 660 FLAG-tagged 660 immunoprecipitate 662 antibody 663 antibodies 664 antibodies 664 coimmunoprecipitations 665 antibodies 665 immunoprecipitation 667 antibodies 667 antibody 668 antibodies 668 immunoprecipitation 669 depletion 671 antibody 671 immunoblotting 671 immunoprecipitation 673 coprecipitated 674 antibody 674 coprecipitated 674 Immunoblot 674 Immunoblot analysis 675 antibody 675 immunoprecipitation 676 antibody 677 in vivo 678 binding assays 678 in vivo 680 coprecipitated 681 antibody 681 Immunoblotting 681 precipitates 682 immunoblotting 682 immunoprecipitation 682 precipitates 683 anti-FLAG 683 immunoblotting 683 precipitates 684 coimmunoprecipitation 684 coimmunoprecipitation experiments 685 immunoprecipitation 685 immunoprecipitation assays 686 antibodies 686 immunoprecipitation 687 immunoprecipitations 687 Western blot 687 Western blot analysis 688 antibodies 688 co-precipitated 688 Western blot 688 Western blot analysis 689 antibody 689 SDS-PAGE 689 Western blot 689 Western blot analysis 693 coimmunoprecipitations 693 immunoprecipitates 695 antibodies 695 immunoprecipitation 696 co-immunoprecipitate 696 immunoprecipitation 697 antibody 697 autoradiography 697 coimmunoprecipitates 697 electrophoresis 697 gel electrophoresis 697 immunoprecipitates 697 polyacrylamide gel 698 bait 698 immunoprecipitation 699 antiserum 699 coimmunoprecipitates 700 antibody 700 coimmunoprecipitates 704 precipitate 706 antibody 706 Immunoprecipitation 707 in vivo 708 in vivo 709 coprecipitation 709 copurified 709 immunoprecipitation 710 immunoprecipitates 712 antibody 712 precipitate 712 Western blots 713 antibody 714 antibodies 714 antibody 714 anti-HA 715 antibody 715 immunoprecipitations 716 Immunoprecipitation 716 pulled down 718 antibodies 718 antibodies against 718 antibody 718 Flag 718 immunoprecipitate 719 pulled down 719 reciprocal experiment 722 coimmunoprecipitation 722 coimmunoprecipitation experiments 722 in vivo 722 two-hybrid 722 two-hybrid screening 722 yeast two-hybrid 724 in vitro 724 in vivo 728 antibodies 728 Antibodies 728 antibodies against 728 Antibodies against 728 coprecipitated 731 antibody 731 coprecipitated 731 immunoblotting 731 Immunoblotting 732 antibody 732 Immunoprecipitation 733 antibodies 733 immunoprecipitation 733 immunoprecipitation experiments 735 affinity purify 735 antibodies 735 immunoprecipitation 735 Western blot 735 Western blot analysis 736 immunoprecipitating 738 antibody 739 Co-immunoprecipitation 739 Co-immunoprecipitation assays 739 GFP 741 antibody 742 antibodies 742 antibodies against 742 precipitate 743 Antibodies 743 Antibodies against 744 Antibodies 744 Antibodies against 745 Antibodies 745 Antibodies against 747 Immunoprecipitating 747 staining 751 antibody 751 In vitro 751 pulled down 753 antibody 753 coimmunoprecipitate 753 in vivo 754 antibodies 754 coimmunoprecipitations 755 antibody 755 immunoprecipitates 757 Immunoprecipitation 757 Western blotting 761 immunoprecipitates 762 immunoprecipitates 766 antibody 767 Coimmunoprecipitation 772 coimmunoprecipitation 773 coprecipitated 773 GFP 774 precipitates 778 antibody 778 immunoprecipitates 779 antibodies 779 coprecipitated 779 in vivo 780 antibody 780 co-precipitated 780 Sepharose 780 Sepharose beads 781 co-precipitated 783 Western blots 784 coprecipitated 784 in vitro 786 immunoprecipitates 787 coprecipitated 787 immunoprecipitates 789 antibody 789 coprecipitated 789 immunoprecipitation 790 antibody 790 coprecipitated 790 immunoprecipitation 792 immunoprecipitation 793 in vivo 795 immunoprecipitates 797 Immunoprecipitation 799 antibody 800 siRNA 801 antibodies 801 immunoprecipitation 801 western blot 801 western blot analyses 803 co-immunoprecipitation 805 co-immunoprecipitation 805 immunoprecipitation 806 coimmunoprecipitation 809 pulse chase 810 antibodies 810 Immunoblotting 811 antibody 812 antibodies 812 antibody 812 immunoblotting 813 antibody 814 antibody 815 coprecipitated 815 Western blots 818 antibodies 819 antibodies 819 antibodies against 820 immunoprecipitate 820 Immunoprecipitation 821 immunoprecipitation 823 Coimmunoprecipitation 823 Coimmunoprecipitation analyses 824 antibody 824 Co-immunoprecipitation 824 mass spectrometry 824 mass spectrometry analysis 825 antibody 825 co-precipitated 826 co-immunoprecipitation 826 co-immunoprecipitation experiments 827 coimmunoprecipitation 827 coimmunoprecipitation analysis 829 antibody 829 co-immunoprecipitation 829 Western blotting 835 Coprecipitation 836 Coprecipitation 837 Coprecipitation 839 co-precipitated 840 co-precipitated 842 coimmunoprecipitation 843 antibody 844 antibody 844 coprecipitated 845 antibody 847 antibody 847 co-purified 847 immunoprecipitation 847 immunoprecipitation assay 848 antibodies 850 co-precipitated 852 coprecipitated 853 FRET 853 FRET analysis 853 in vivo 857 antibodies 858 co-precipitate 860 immunoprecipitates 861 antibody 861 immunoprecipitation 863 immunoprecipitates 863 immunoprecipitation 863 immunoprecipitation experiments 864 copurified 865 antibody 865 Immunoprecipitation 866 antibody 866 coprecipitated 866 Immunoprecipitates 868 antibody 868 co-precipitation 868 immunoprecipitation 870 co-precipitated 871 co-precipitated 872 antibody 873 antibody 876 antibody 877 antibodies 877 antibody 877 immunoblotting 878 immunoprecipitate 880 sequenced 881 coprecipitated 881 precipitates 882 coprecipitating 882 immunoprecipitations 885 precipitates 888 Co-IP 891 antibody 891 anti-Flag 891 immunoprecipitate 892 antibody 892 immunoprecipitate 893 Antibodies 893 GFP 894 Co-immunoprecipitation 895 coimmunoprecipitation 895 coimmunoprecipitation assays 897 knockout 898 western blotting 900 coprecipitated 901 antibody 901 anti-GFP 901 coimmunoprecipitation 901 western blotting 903 immunoprecipitates 904 immunoprecipitates 905 in vivo 906 immunoprecipitate 909 antibodies 909 in vitro-translated 910 coprecipitated 911 antibodies 911 coprecipitated 912 antiserum 914 immunoprecipitates 915 antibodies 915 SDS-PAGE 916 immunoprecipitation 917 antibodies 919 antibodies 919 polyacrylamide gel 922 antibodies 922 antibodies against 922 immunoprecipitation 922 immunoprecipitation experiments 923 immunoprecipitates 923 staining 924 coprecipitated 925 antibody 925 in vivo 926 antibodies 926 immunoprecipitates 927 coimmunoprecipitation 929 coimmunoprecipitation 931 Co-immunoprecipitation 932 copurification 933 antibody 934 affinity column 934 immunoblot 934 immunoblot analysis 935 immunoblots 935 Immunoprecipitates 936 immunoprecipitation 936 precipitates 937 Immunoprecipitations 937 precipitate 939 antibodies 939 co-precipitated 940 immunoprecipitates 941 antibody 942 precipitate 943 antibodies 945 antibody 946 antibodies 946 antibody 947 antibody 947 coprecipitate 947 Western blotting 948 in vitro 950 coimmunoprecipitation 951 Co-immunoprecipitation 952 HA-tagged 952 in vivo 953 antibodies 954 antiserum 954 immunoprecipitates 955 antibody 955 immunoprecipitates 956 antibody 956 immunoprecipitates 958 antibodies 958 Western blotting 959 antibodies 959 anti-HA 959 Immunoprecipitations 959 Western blotting 963 antibody 963 coprecipitated 968 antibodies 968 fusion protein 969 in vitro 969 in vivo 970 in vivo 971 immunoprecipitate 972 antibodies 972 Immunoprecipitates 973 antibodies 973 Coimmunoprecipitation 974 Coimmunoprecipitation 975 in vivo 977 antibody 979 immunoprecipitates 982 antibody 983 copurification 983 immunoprecipitation 983 mass spectrometry 983 RSA 983 Western blot 984 antibodies 984 antibodies against 985 knockin 985 titration 986 Immunoprecipitation 986 Immunoprecipitation analyses 990 Coimmunoprecipitation 990 Coimmunoprecipitation results 997 coprecipitated 1001 HA-tagged 1001 precipitates 1002 coprecipitated 1007 precipitate 1010 immunoprecipitation 1010 immunoprecipitation experiments 1011 Immunoprecipitation 1011 Immunoprecipitation experiments 1012 Co-immunoprecipitation 1013 antibody 1013 co-precipitation 1013 Western blotting 1014 coprecipitated 1014 immunoprecipitations 1015 coprecipitated 1015 immunoprecipitations 1016 coprecipitated 1016 immunoprecipitations 1017 coprecipitation 1018 coprecipitation 1024 antibody 1025 antibody 1025 immunoprecipitates 1027 antibodies 1027 antibodies against 1027 immunoprecipitation 1029 FLAG 1029 precipitates 1030 western blotting 1031 Flag 1031 immunoaffinity 1031 purification 1031 SDS-PAGE 1032 binding assays 1032 immunoprecipitations 1032 in vitro 1032 in vitro binding assays 1032 in vitro translated 1033 antibodies 1033 immunoprecipitations 1033 in vivo 1034 antibodies 1034 immunoprecipitate 1034 immunoprecipitates 1034 immunoprecipitations 1034 in vivo 1035 antibodies 1035 antibodies against 1035 anti-Flag 1035 coprecipitated 1035 Flag-tagged 1035 immunoprecipitating 1036 antibodies 1036 Coimmunoprecipitation 1037 In vivo 1043 coimmunoprecipitation 1043 coimmunoprecipitation experiments 1047 antibody 1047 coprecipitated 1047 immunoprecipitation 1048 coprecipitate 1050 immunoblotting 1050 immunoprecipitates 1054 antibody 1054 immunoblotting 1054 immunoprecipitates 1055 immunoblotting 1055 immunoprecipitates 1056 coimmunoprecipitates 1057 Immunoblot 1057 Immunoblot analysis 1057 immunoprecipitates 1059 In vivo 1060 Co-precipitation 1061 antibody 1061 coprecipitation 1061 Immunoblots 1061 immunoprecipitation 1062 antibody 1062 coimmunoprecipitation 1062 immunoprecipitation 1064 coimmunoprecipitates 1066 antibody 1070 coimmunoprecipitation 1070 coimmunoprecipitation experiments 1072 antibodies 1072 immunoblotting 1073 antibodies 1073 immunoblotting 1074 immunoprecipitates 1074 precipitates 1075 antibody 1075 coprecipitated 1075 immunoprecipitates 1078 coimmunoprecipitation 1080 immunoprecipitates 1083 TAP 1085 antibodies 1085 immunoprecipitates 1086 mass spectrometry 1086 MS/MS 1086 MS/MS analysis 1086 staining 1086 tandem mass spectrometry 1086 TAP 1087 antibody 1087 immunoprecipitation 1088 Coimmunoprecipitations 1090 antibody 1091 antibody 1091 immunoprecipitates 1093 coimmunoprecipitation 1097 immunoblot 1097 immunoblot analysis 1098 Coimmunoprecipitation 1098 Coimmunoprecipitation experiments 1099 Coimmunoprecipitation 1099 Coimmunoprecipitation assays 1100 coimmunoprecipitates 1100 Immunoblots 1102 immunoblotting 1102 immunoprecipitates 1103 immunoblotting 1103 immunoprecipitates 1104 immunoblots 1104 immunoprecipitate 1109 antibodies 1109 antibodies against 1109 antibody 1113 antibody 1113 immunoprecipitates 1115 immunoprecipitates 1118 antibody 1118 immunoprecipitation 1121 Coimmunoprecipitation 1126 co-precipitated 1126 in vivo 1127 copurify 1128 copurify 1128 Flag 1128 Flag-tagged 1128 immunopurification 1129 coimmunoprecipitates 1131 antibodies 1131 coimmunoprecipitation 1131 coimmunoprecipitation experiments 1131 Immunoprecipitates 1131 in vivo 1133 antibodies 1134 Co-immunoprecipitation 1135 fusion protein 1136 fusion protein 1136 immunoprecipitation 1137 antibody 1137 anti-FLAG 1137 anti-FLAG antibody 1137 anti-HA 1137 FLAG 1137 in vitro 1137 in vitro translated 1138 coimmunoprecipitation 1140 co-precipitation 1140 in vivo 1140 Western blotting 1142 immunoprecipitate 1144 antibodies 1144 anti-HA 1145 coimmunoprecipitation 1145 coimmunoprecipitation experiments 1146 antibodies 1146 anti-FLAG 1146 anti-HA 1146 FLAG 1146 immunoprecipitation 1146 in vitro 1147 coprecipitated 1148 antibody 1148 precipitate 1149 antibody 1149 anti-HA 1149 coimmunoprecipitation 1149 Immunoblotting 1150 chromatography 1150 immunoaffinity 1150 immunoaffinity chromatography 1151 Immunoblotting 1152 antibodies 1153 co-precipitated 1153 two-hybrid 1154 coprecipitated 1155 coprecipitated 1156 coprecipitated 1158 anti-FLAG 1158 immunoprecipitates 1159 co-immunoprecipitates 1160 co-immunoprecipitation 1161 antibody 1161 precipitate 1165 FLAG 1165 FLAG-tagged 1165 HA-tagged 1167 co-precipitated 1168 co-precipitated 1171 antibody 1171 anti-FLAG 1171 anti-FLAG antibody 1171 immunoprecipitation 1171 Western blot 1171 Western blot analysis 1172 antibody 1172 in vitro 1173 antibodies 1173 immunoprecipitates 1173 immunoprecipitation 1174 anti-FLAG 1174 FLAG 1175 anti-FLAG 1175 FLAG 1176 peptide sequencing 1176 western blot 1176 western blot analysis 1178 Immunoprecipitation 1178 Immunoprecipitation analysis 1180 co-immunoprecipitation 1180 immunoprecipitation 1180 Immunoprecipitation 1184 co-immunoprecipitation 1185 anti-FLAG 1185 fusion protein 1185 Western blot 1185 Western blot analysis 1186 Co-immunoprecipitation 1188 Coimmunoprecipitation 1189 antibody 1189 anti-FLAG 1189 anti-FLAG antibody 1189 coprecipitates 1191 antibodies 1191 anti-HA 1191 immunoprecipitation 1191 immunoprecipitation experiments 1191 precipitate 1192 immunoprecipitates 1192 in vivo 1193 co-immunoprecipitation 1193 co-immunoprecipitation experiments 1193 HA-tagged 1195 immunoprecipitates 1196 nocodazole 1198 coimmunoprecipitation 1199 coimmunoprecipitation 1199 coimmunoprecipitation experiments 1202 antibodies 1202 coimmunoprecipitation 1202 coimmunoprecipitation assay 1202 HA-tagged 1202 immunoprecipitation 1203 HA-tagged 1203 immunoprecipitation 1204 coimmunoprecipitation 1204 coimmunoprecipitation experiments 1205 co-immunoprecipitation 1209 co-precipitated 1210 in vivo 1211 antibody 1211 Co-immunoprecipitation 1212 antibodies 1212 antibody 1212 anti-FLAG 1212 anti-FLAG antibody 1212 immunoprecipitation 1213 immunoblotting 1213 immunoprecipitates 1214 antibodies 1214 anti-HA 1214 co-precipitated 1214 HA-tagged 1214 In vitro 1215 HA-tagged 1216 antibodies 1216 co-immunoprecipitation 1216 immunoblot 1217 co-precipitated 1223 antibody 1223 anti-HA 1223 HA-tagged 1224 antibody 1224 anti-HA 1224 HA-tagged 1225 co-immunoprecipitation 1225 co-immunoprecipitation experiments 1226 co-immunoprecipitation 1226 co-immunoprecipitation experiments 1227 anti-HA 1227 immunopurified 1228 antibody 1228 anti-HA 1228 FLAG-tagged 1228 immunoprecipitate 1231 antibodies 1231 anti-HA 1231 copurified 1231 Immunoblotting 1233 in vitro 1236 antibodies 1237 antibody 1237 depletion 1238 anti-FLAG 1238 FLAG-tagged 1238 immunoprecipitation 1238 Western blot 1239 anti-FLAG 1239 FLAG-tagged 1239 Immunoprecipitates 1239 immunoprecipitation 1239 Western blot 1240 antibody 1240 Western blot 1241 binding assay 1241 in vitro 1241 in vitro translated 1243 HA-tagged 1243 immunoprecipitates 1244 anti-HA 1244 immunoprecipitate 1245 immunoprecipitates 1246 immunoprecipitates 1246 reciprocal experiments 1247 immunoprecipitates 1248 antibodies 1248 coprecipitated 1248 immunoprecipitate 1249 coprecipitated 1250 antibodies 1250 anti-HA 1250 pulled down 1252 FLAG-tagged 1252 HA-tagged 1253 anti-HA 1253 immunoprecipitates 1254 antibody 1254 anti-FLAG 1254 anti-FLAG antibody 1254 FLAG 1254 precipitates 1255 antibody 1255 anti-FLAG 1255 anti-FLAG antibody 1255 FLAG-tagged 1255 fusion protein 1256 antibody 1256 anti-FLAG 1256 anti-FLAG antibody 1256 FLAG-tagged 1256 HA-tagged 1257 immunoprecipitates 1258 antibodies 1258 anti-HA 1258 Co-immunoprecipitation 1258 in vitro 1258 prey 1259 immunoprecipitates 1260 immunoblot 1260 immunoprecipitate 1261 anti-GFP 1261 coprecipitates 1261 immunoblots 1263 co-precipitated 1263 HA-tagged 1266 in vitro 1267 FLAG-tagged 1267 immunoprecipitates 1267 Immunoprecipitation 1267 Immunoprecipitation analyses 1269 coprecipitated 1269 in vivo 1269 pulled down 1270 Coimmunoprecipitation 1271 fusion protein 1272 co-immunoprecipitation 1272 co-immunoprecipitation analysis 1272 Flag-tagged 1272 in vivo 1273 fusion protein 1274 Flag-tagged 1277 mass spectrometry 1277 SDS-PAGE 1277 sequence analysis 1277 staining 1277 staining method 1278 coimmunoprecipitation 1278 coimmunoprecipitation experiments 1278 HA-tagged 1280 Immunoprecipitation 1280 Immunoprecipitation analyses 1282 Western analysis 1283 co-immunoprecipitation 1283 co-immunoprecipitation assay 1284 immunoblots 1284 immunoprecipitates 1286 pulled down 1287 pulled down 1288 pulled down 1289 pull down 1290 co-immunoprecipitation 1290 co-immunoprecipitation experiments 1291 coprecipitated 1292 Coimmunoprecipitation 1295 Co-immunoprecipitation 1296 binding assays 1296 co-IP assays 1296 in vitro 1296 in vitro binding assays 1296 in vivo 1298 coimmunoprecipitation 1298 in vitro 1298 in vitro translated 1298 SDS-PAGE 1298 two-hybrid 1298 two-hybrid system 1298 Western blotting 1298 yeast two-hybrid 1298 yeast two-hybrid system 1299 in vitro 1300 antibody 1300 anti-FLAG 1300 anti-FLAG antibody 1300 FLAG-tagged 1300 Western blotting 1301 anti-HA 1301 immunoprecipitates 1302 antibody 1302 anti-FLAG 1302 anti-FLAG antibody 1302 FLAG 1302 FLAG-tagged 1302 HA-tagged 1302 precipitates 1303 coprecipitated 1305 anti-HA 1305 Immunoprecipitation 1306 immunoprecipitate 1306 mass spectrometric analysis 1307 immunoprecipitate 1307 Mass spectrometry 1308 coimmunoprecipitation 1308 coimmunoprecipitation experiments 1308 Flag-tagged 1308 green fluorescent protein 1309 antibody 1309 anti-HA 1309 coprecipitated 1309 immunoprecipitation 1309 precipitate 1309 precipitates 1309 pulled down 1311 immunoprecipitate 1311 immunoprecipitation 1311 western blotting 1312 Coimmunoprecipitation 1312 Coimmunoprecipitation assay 1313 coimmunoprecipitation 1313 coimmunoprecipitation experiment 1314 immunoblotting 1317 antibody 1317 anti-Flag 1317 anti-HA 1317 coprecipitated 1317 Western blot 1317 Western blot analysis 1318 coprecipitated 1319 coimmunoprecipitation 1319 In vitro 1320 co-immunoprecipitation 1320 In vitro 1321 immunoprecipitate 1322 coprecipitated 1322 immunoprecipitation 1323 antibody 1324 antibodies 1324 Flag-tagged 1324 immunoblotting 1324 immunoprecipitation 1326 antibodies 1326 Immunoprecipitation 1326 Western blotting 1330 in vitro 1330 in vivo 1331 co-immunoprecipitation 1331 two-hybrid 1331 yeast two-hybrid 1332 TAP 1338 antibody 1338 coprecipitated 1338 two-hybrid 1338 two-hybrid assays 1338 yeast two-hybrid 1338 yeast two-hybrid assays 1340 coprecipitated 1342 antibody 1342 anti-HA 1342 immunoblot 1342 immunoprecipitate 1342 immunoprecipitation 1344 co-immunoprecipitation 1344 co-immunoprecipitation experiments 1344 in vivo 1347 Co-immunoprecipitation 1347 Co-immunoprecipitation experiments 1349 antibody 1349 anti-GFP 1349 anti-GFP antibody 1349 pull down 1349 Western blotting 1351 two-hybrid 1352 coprecipitates 1352 Immunoprecipitation 1353 co-IP analysis 1353 in vivo 1354 antibody 1354 anti-GFP 1354 anti-GFP antibody 1354 anti-HA 1354 co-precipitated 1354 immunoprecipitation 1354 Immunoprecipitation 1354 precipitates 1355 co-precipitated 1355 Immunoblotting 1356 co-immunoprecipitate 1357 BRET 1357 BRET assays 1357 co-immunoprecipitation 1357 co-immunoprecipitation assays 1358 co-precipitate 1360 co-precipitated 1360 FLAG-tagged 1360 GST 1360 immunoprecipitation 1363 immunoprecipitations 1363 Western blot 1363 Western blot analysis 1364 Coimmunoprecipitation 1365 antibody 1365 Co-immunoprecipitation 1365 in vitro 1370 Coimmunoprecipitation 1371 immunoprecipitates 1372 immunoprecipitates 1373 coimmunoprecipitation 1374 anti-Flag 1374 Flag-tagged 1374 immunoprecipitates 1375 immunoprecipitate 1376 in vivo 1378 coimmunoprecipitation 1378 coimmunoprecipitation assays 1378 GFP 1378 in vivo 1382 antibody 1382 anti-GFP 1382 anti-GFP antibody 1382 anti-HA 1382 coimmunoprecipitation 1382 two-hybrid 1384 co-immunoprecipitate 1384 Immunoblotting 1384 immunoprecipitates 1386 copurified 1387 copurified 1388 anti-Flag 1389 immunoprecipitation 1392 antibodies 1392 pulled down 1393 coimmunoprecipitation 1393 coimmunoprecipitation analysis 1394 antibody 1394 co-precipitated 1394 epitope tag 1394 HA-tagged 1394 in vivo 1396 coimmunoprecipitation 1398 Co-immunoprecipitation 1402 immunoprecipitations 1405 antibody 1405 anti-HA 1407 coimmunoprecipitation 1407 coimmunoprecipitation assays 1407 two-hybrid 1411 antibody 1411 HA-tagged 1413 FLAG-tagged 1413 immunoprecipitation 1413 immunoprecipitation experiment 1414 antibody 1414 anti-GFP 1414 anti-GFP antibody 1414 coprecipitated 1414 fusion protein 1414 Immunoprecipitation 1415 antibodies 1415 anti-FLAG 1415 fusion protein 1415 precipitate 1417 co-immunoprecipitation 1420 antibody 1420 FLAG-tag 1420 FLAG-tagged 1420 immunoprecipitates 1420 monoclonal antibody 1421 FLAG 1421 immunoprecipitates 1424 antibody 1424 anti-GFP 1424 anti-GFP antibody 1424 anti-HA 1424 coimmunoprecipitates 1424 immunoblotting 1424 Immunoprecipitation 1426 coimmunoprecipitate 1427 co-immunoprecipitate 1427 two-hybrid 1427 two-hybrid screen 1427 yeast two-hybrid 1427 yeast two-hybrid screen 1429 immunoprecipitates 1430 co-immunoprecipitation 1430 co-immunoprecipitation experiments 1430 HA-tagged 1434 co-immunoprecipitation 1437 in vitro 1438 FLAG 1438 FLAG tags 1439 co-immunoprecipitate 1442 co-immunoprecipitation 1442 co-immunoprecipitation assay 1443 antibody 1445 immunoprecipitate 1446 immunoprecipitate 1447 immunoprecipitate 1448 coimmunoprecipitate 1449 antibody 1449 anti-FLAG 1449 anti-FLAG antibody 1449 Western blot 1451 Immunoprecipitation 1451 mass spectrometry 1454 antibody 1454 coexpression 1454 co-precipitate 1454 HA-tagged 1456 cotransfection 1456 cotransfection experiments 1461 Purification 1462 in vitro 1463 in vivo 1467 co-precipitated 1468 antibodies 1468 anti-FLAG 1468 FLAG-tagged 1468 immunoprecipitation 1469 antibodies 1469 anti-FLAG 1469 anti-GFP 1469 FLAG-tagged 1469 immunoprecipitation 1470 antibodies 1470 anti-FLAG 1470 co-immunoprecipitation 1470 immunoblot 1470 immunoblot analysis 1473 coimmunoprecipitation 1473 coimmunoprecipitation assays 1476 colocalize 1476 coprecipitate 1477 colocalize 1477 coprecipitate 1477 purification 1478 copurify 1482 copurified 1482 mass spectrometry 1487 copurify 1489 immunoprecipitates 1490 precipitate 1491 anti-FLAG 1491 FLAG-tagged 1492 anti-FLAG 1492 FLAG-tagged 1492 immunoprecipitates 1494 in vivo 1495 antibody 1495 immunoprecipitates 1496 co-immunoprecipitation 1496 Western blot 1496 Western blot analysis 1497 antibody 1497 co-immunoprecipitation 1497 Western blot 1497 Western blot analysis 1498 pull down 1501 immunoblot 1503 binding assays 1503 In vitro 1503 In vitro binding assays 1504 antibodies 1504 antibody 1504 anti-GFP 1504 GFP 1504 immunoprecipitation 1505 anti-Flag 1505 co-precipitated 1506 coprecipitated 1509 co-immunoprecipitation 1509 FLAG-tagged 1510 Deletion mutational analyses 1510 mutational analyses 1511 Deletion mutational analyses 1511 mutational analyses 1516 immunoblotting 1516 in vivo 1517 Co-IP 1517 Co-IP assays 1519 HA-tagged 1520 in vivo 1526 Co-immunoprecipitation 1527 coimmunoprecipitations 1530 antibody 1531 coimmunoprecipitates 1531 fusion protein 1531 immunoprecipitates 1532 coprecipitated 1532 fusion protein 1533 antibodies 1533 anti-HA 1533 Coexpression 1533 coimmunoprecipitation 1540 anti-Flag 1540 Flag 1540 immunoprecipitates 1540 Western blot 1541 in vitro 1541 SPA 1542 in vitro 1545 antibodies 1545 antibody 1545 anti-FLAG 1545 anti-FLAG antibody 1545 anti-HA 1545 FLAG-tagged 1545 HA-tagged 1545 Immunoblots 1546 immunoblot 1549 coimmunoprecipitation 1549 FLAG-tagged 1549 Immunoprecipitation 1550 coimmunoprecipitation 1550 FLAG-tagged 1550 immunoprecipitation 1552 antibody 1553 coprecipitated 1555 immunoprecipitates 1556 coprecipitate 1557 antibodies 1557 anti FLAG 1557 co-immunoprecipitation 1557 FLAG 1557 immunoprecipitations 1558 In vitro 1558 In vitro translated 1562 anti-FLAG 1562 immunoprecipitation 1562 pulldown 1563 antibody 1563 anti-HA 1563 HA-tagged 1564 antibody 1564 anti-HA 1564 HA-tagged 1564 reciprocal experiment 1565 antibody 1566 coimmunoprecipitation 1569 co-immunoprecipitation 1569 co-immunoprecipitation assays 1570 copurified 1571 antibodies 1571 antibody 1571 anti-GFP 1571 anti-GFP antibody 1571 GFP 1571 immunoprecipitation 1571 immunoprecipitation experiments 1571 precipitates 1571 purification 1571 Western blot 1571 Western blot analysis 1574 anti-FLAG 1574 Immunoprecipitates 1575 co-precipitated 1575 immunoprecipitating 1576 in vivo 1576 two-hybrid 1577 co-precipitated 1578 Co-immunoprecipitations 1578 in vivo 1579 in vivo 1580 immunoprecipitates 1581 anti-FLAG 1581 immunoprecipitations 1582 co-immunoprecipitates 1583 antibodies 1583 antibodies against 1583 co-precipitate 1584 co-immunoprecipitation 1584 co-immunoprecipitation experiments 1584 in vivo 1585 anti-HA 1585 immunoprecipitates 1586 HA-tagged 1589 co-immunoprecipitation 1589 fusion protein 1589 GFP 1591 in vitro 1593 antibodies 1593 anti-HA 1593 immunoprecipitation 1595 immunoprecipitates 1596 immunoprecipitates 1597 immunoprecipitates 1598 Immunoprecipitation 1598 in vivo 1599 co-precipitated 1600 co-immunoprecipitation 1600 co-immunoprecipitation analysis 1600 FLAG-tagged 1601 antibody 1601 anti-Flag 1601 anti-HA 1601 Flag-tagged 1601 HA-tagged 1601 immunoprecipitates 1601 Western blot 1601 Western blot analysis 1602 Immunoprecipitation 1602 Immunoprecipitation experiments 1603 co-immunoprecipitation 1603 two-hybrid 1603 two-hybrid experiments 1603 yeast two-hybrid 1603 yeast two-hybrid experiments 1604 antibody 1604 Flag-tagged 1604 immunoprecipitation 1605 antibodies 1605 antibody 1605 immunoprecipitates 1610 anti HA 1610 antiserum 1610 coimmunoprecipitations 1612 co-immunoprecipitations 1617 antibodies 1617 anti-HA 1617 co-precipitated 1617 Immunoblotting 1617 precipitates 1619 FLAG 1621 antibodies 1621 antibody 1621 anti-FLAG 1621 anti-FLAG antibody 1621 immunoprecipitate 1621 Western blotting 1623 fingerprinting 1623 FLAG-tagged 1623 mass spectrometry 1623 Peptide mass fingerprinting 1623 tandem mass spectrometry 1623 X-ray 1626 immunoprecipitations 1627 antibodies 1627 Western blot 1628 antibodies 1629 co-purified 1629 in vivo 1629 mass spectrometry 1629 western blot 1630 coexpression 1631 antibodies 1631 GFP 1631 Immunoprecipitation 1631 in vivo 1633 immunoprecipitates 1634 immunoblotting 1634 immunoblotting assays 1634 immunoprecipitation 1635 immunoblotting 1635 immunoblotting assays 1635 immunoprecipitation 1636 antibody 1636 immunoblotting 1636 immunoblotting assay 1636 immunoprecipitation 1637 antibody 1637 coprecipitated 1637 FLAG 1637 pulled down 1638 binding assay 1638 in vivo 1639 antibody 1639 Coimmunoprecipitation 1639 immunoprecipitation 1639 precipitates 1639 Western blot 1642 antibody 1642 anti-HA 1642 Co-IP 1642 western blots 1642 western blotting 1644 antibody 1644 coimmunoprecipitation 1644 coimmunoprecipitation assay 1644 FLAG 1644 in vivo 1645 anti-HA 1645 immunoblotting 1647 coprecipitated 1648 coprecipitated 1650 coimmunoprecipitations 1650 immunoprecipitates 1651 antibodies 1651 anti-FLAG 1652 antibodies 1652 ESR 1652 GST 1652 GST-tagged 1652 pull down 1653 antibody 1653 anti-HA 1654 antibody 1654 immunoprecipitation 1655 antibody 1655 co-IP experiment 1655 Flag 1656 co-precipitated 1656 GFP 1657 co-precipitated 1657 GFP 1658 co-precipitated 1660 coprecipitated 1661 pulled down 1662 antibody 1662 Immunoprecipitation 1662 western blot 1663 antibody 1666 coimmunoprecipitation 1666 coimmunoprecipitation assay 1667 antibody 1667 anti-FLAG 1667 anti-FLAG antibody 1667 anti-HA 1667 FLAG-tagged 1667 HA-tagged 1667 Western blotting 1668 antibody 1668 anti-HA 1668 HA-tagged 1668 immunoprecipitation 1668 Immunoprecipitation 1669 antibodies 1669 antibodies against 1669 antibody 1669 Flag 1669 immunoprecipitate 1670 anti-HA 1670 pulled down 1676 coimmunoprecipitation 1676 coimmunoprecipitation experiments 1676 in vivo 1676 two-hybrid 1676 two-hybrid screening 1676 yeast two-hybrid 1678 immunoprecipitates 1679 antibody 1679 anti-GFP 1679 anti-GFP antibody 1679 immunoprecipitate 1680 antibodies 1680 anti-FLAG 1680 coprecipitated 1681 antibodies 1681 anti-FLAG 1681 coprecipitated 1681 immunoprecipitation 1681 immunoprecipitation assays 1682 antibodies 1684 anti-FLAG 1684 coprecipitated 1684 immunoprecipitates 1685 antibodies 1685 anti-Flag 1685 FLAG 1685 FLAG-tagged 1685 immunoblotting 1685 Immunoprecipitation 1686 antibodies 1686 antibodies against 1686 FLAG 1686 FLAG-tagged 1686 immunoblotting 1687 FLAG-tagged 1689 antibodies 1689 FLAG-tagged 1691 antibody 1691 Co-immunoprecipitation 1693 prey 1694 coimmunoprecipitates 1696 Co-IP 1696 Co-IP assays 1697 antibodies 1697 Co-IP 1697 Co-IP assays 1697 Flag 1699 coprecipitation 1700 coprecipitated 1700 precipitate 1701 co-immunoprecipitation 1701 preys 1702 antibody 1702 anti-HA 1702 coimmunoprecipitation 1702 coimmunoprecipitation assay 1702 coprecipitated 1702 in vitro 1705 antibodies 1706 Immunoprecipitation 1706 western blot 1706 western blot analysis 1707 antibody 1707 anti-Flag 1707 coprecipitated 1709 coprecipitate 1710 coprecipitation 1711 coprecipitates 1712 coprecipitated 1712 Western blot 1712 Western blot analyses 1713 co-immunoprecipitation 1714 co-immunoprecipitation 1714 co-precipitates 1714 immunoprecipitation 1715 immunoprecipitation 1717 anti-FLAG 1717 coprecipitated 1718 Coimmunoprecipitation 1719 Coimmunoprecipitation 1720 antibody 1720 co-immunoprecipitation 1720 in vitro 1721 green fluorescent protein 1721 in vivo 1724 co-precipitated 1726 FLAG-tagged 1727 anti-FLAG 1727 co-immunoprecipitation 1727 immunoprecipitation 1727 precipitates 1728 immunoprecipitation 1735 coprecipitations 1737 Coexpression 1737 FLAG-tagged 1738 immunoprecipitates 1739 immunoprecipitates 1739 Western blot 1740 in vitro 1741 Coimmunoprecipitation 1744 coimmunoprecipitation 1744 epitope tags 1746 epitope tags 1747 epitope tags 1747 two-hybrid 1747 two-hybrid assay 1747 yeast two-hybrid 1747 yeast two-hybrid assay 1749 immunoprecipitates 1750 anti-GFP 1750 anti-HA 1751 Interaction assays 1752 antibody 1752 anti-FLAG 1752 anti-FLAG antibody 1752 anti-HA 1752 Interaction assays 1752 pulldown 1752 pull-down 1752 pull-down assays 1752 pulldown assays 1756 antibody 1756 anti-FLAG 1756 anti-FLAG antibody 1756 immunoprecipitation 1757 antibody 1757 anti-FLAG 1757 anti-FLAG antibody 1757 HA-tagged 1757 immunoblotting 1757 immunoprecipitation 1758 antibody 1758 anti-FLAG 1758 anti-FLAG antibody 1758 HA-tagged 1758 immunoblotting 1758 immunoprecipitation 1761 antibody 1761 anti-HA 1761 co-immunoprecipitation 1761 co-immunoprecipitation assay 1761 reciprocal co-immunoprecipitation 1761 reciprocal co-immunoprecipitation assay 1762 FLAG-tagged 1762 HA-tagged 1762 immunoblot 1762 immunoblot analysis 1762 Immunoprecipitation 1763 antibodies 1763 immunoprecipitates 1765 in vivo 1774 co-immunoprecipitation 1774 co-immunoprecipitation assays 1774 two-hybrid 1774 yeast two-hybrid 1777 co-immunoprecipitation 1777 co-IP experiments 1777 epitope tags 1777 two-hybrid 1777 two-hybrid assays 1777 yeast two-hybrid 1777 yeast two-hybrid assays 1779 coprecipitated 1780 In vitro 1781 in vitro 1782 In vivo 1785 coimmunoprecipitation 1785 Flag 1785 immunoprecipitation 1786 purification 1787 Coimmunoprecipitation 1789 in vivo 1790 antibodies 1791 coimmunoprecipitation 1792 precipitates 1793 antibody 1793 anti-GFP 1793 anti-GFP antibody 1793 coprecipitated 1793 Immunoprecipitation 1794 antibody 1795 coimmunoprecipitation 1795 coimmunoprecipitation assay 1795 Western blot 1796 TAP 1797 TAP 1798 Co-immunoprecipitation 1798 co-precipitated 1798 immunoprecipitations 1798 precipitate 1799 antibodies 1799 anti-FLAG 1799 anti-HA 1799 coimmunoprecipitation 1799 immunoprecipitation 1799 immunoprecipitation assays 1800 in vivo 1801 antibody 1801 anti-HA 1801 immunoprecipitates 1802 antibody 1802 immunoprecipitate 1803 co-precipitated 1808 co-precipitate 1810 immunoprecipitations 1818 coimmunoprecipitation 1818 coimmunoprecipitation experiments 1820 coimmunoprecipitation 1820 coimmunoprecipitation experiments 1821 FLAG-tag 1821 FLAG-tagged 1822 copurified 1824 coimmunoprecipitation 1825 immunoprecipitation 1830 Flag-tagged 1831 coexpression 1831 coimmunoprecipitates 1834 anti-Flag 1834 immunoprecipitation 1835 Immunoprecipitation 1836 immunoprecipitation 1837 Mass spectrometric analysis 1837 SDS-PAGE 1839 coimmunoprecipitate 1839 Flag-tagged 1841 antibody 1841 co-precipitation 1841 immunoprecipitation 1842 co-precipitation 1842 Immunoprecipitation 1843 antibody 1843 co-precipitation 1843 immunoprecipitation 1845 antibody 1845 coprecipitated 1845 Flag 1846 antibody 1846 anti-FLAG 1846 anti-FLAG antibody 1846 FLAG-tagged 1848 Co-immunoprecipitation 1848 Co-immunoprecipitation assay 1848 two-hybrid 1848 two-hybrid result 1848 yeast two-hybrid 1848 yeast two-hybrid result 1850 antibody 1850 anti-GFP 1850 anti-GFP antibody 1851 antibody 1851 anti-HA 1851 co-immunoprecipitate 1851 HA-tagged 1855 antibody 1855 coimmunoprecipitation 1855 Coimmunoprecipitation 1855 coimmunoprecipitation experiments 1855 epitope tag 1855 in vivo 1856 coprecipitated 1856 Immunoprecipitation 1856 Immunoprecipitation analysis 1857 HA-tagged 1857 immunoprecipitation 1859 antibody 1859 anti-FLAG 1859 anti-FLAG antibody 1860 antibody 1860 FLAG 1860 FLAG-tagged 1861 antibody 1863 antibody 1863 anti-HA 1863 HA-tagged 1863 immunoblot 1863 immunoblot analysis 1863 immunodetection 1863 immunoprecipitation 1863 monoclonal antibody 1863 reciprocal experiments 1865 coprecipitates 1866 co-IP experiments 1866 pulls down 1867 co-IP experiments 1867 pulls down 1868 in vitro-translated 1870 coprecipitated 1871 in vitro 1873 Co-IP 1874 Co-IP 1877 coprecipitated 1878 antibody 1878 Flag 1878 immunoprecipitation 1879 co-IP assay 1881 antibody 1881 anti-Flag 1881 immunoprecipitate 1881 immunoprecipitation 1882 antibody 1882 anti-HA 1882 immunoprecipitate 1883 Co-immunoprecipitation 1883 Co-immunoprecipitation assay 1884 co-immunoprecipitation 1884 co-immunoprecipitation assay 1885 antibodies 1885 anti-GFP 1887 antibody 1887 anti-GFP 1887 anti-GFP antibody 1887 HA-tagged 1890 immunoprecipitation 1890 IP assay 1891 immunoprecipitates 1891 mass spectrometry 1892 antibody 1892 Flag 1892 Immunoprecipitation 1892 Immunoprecipitation assays 1894 antibody 1894 anti-FLAG 1895 Coimmunoprecipitation 1895 Coimmunoprecipitation assays 1895 in vivo 1896 antibody 1896 anti-GFP 1896 anti-GFP antibody 1896 Coimmunoprecipitation 1896 Co-IP 1896 Co-IP experiments 1896 pulled down 1898 immunoprecipitate 1899 co-precipitate 1900 co-precipitated 1900 HA-tagged 1900 in vitro 1904 antibody 1904 Co-immunoprecipitation 1904 fusion protein 1904 immobilized 1904 in vitro 1904 monoclonal antibody 1905 antibody 1905 anti-GFP 1905 anti-GFP antibody 1905 Co-immunoprecipitation 1906 co-immunoprecipitation 1906 co-immunoprecipitation assays 1906 pulled down 1907 affinity chromatography 1907 anti-FLAG 1907 chromatography 1907 FLAG 1907 His-tag 1907 IMAC 1907 immobilized 1907 immobilized metal affinity chromatography 1907 immunoprecipitation 1907 Mass spectrometry 1908 anti-HA 1908 immunoprecipitates 1909 immunoprecipitates 1910 antibody 1910 anti-Flag 1910 anti-HA 1910 immunoblotting 1910 immunoprecipitates 1910 immunoprecipitation 1911 antibody 1911 anti-GFP 1911 anti-GFP antibody 1911 mass spectrometric analysis 1911 purification 1912 Coimmunoprecipitation 1913 anti-GFP 1914 Immunoprecipitations 1915 co-immunoprecipitation 1916 co-precipitate 1917 antiserum 1917 coimmunoprecipitation 1917 precipitates 1917 Western blotting 1920 coimmunoprecipitates 1921 In vivo 1922 copurification 1923 affinity chromatography 1923 chromatography 1923 Co-purification 1924 antibody 1924 anti-HA 1924 HA-tagged 1924 immunoprecipitation 1925 antiserum 1925 immunoprecipitates 1927 antibody 1927 monoclonal antibody 1928 antibody 1928 coprecipitation 1929 antibody 1929 immunoprecipitate 1929 Western blot 1929 Western blot assay 1932 immunoprecipitation 1933 Coprecipitation 1934 coprecipitated 1934 fusion protein 1935 coprecipitated 1936 coprecipitate 1936 immunoprecipitation 1936 immunoprecipitations 1936 reciprocal experiment 1937 coprecipitate 1937 immunoprecipitation 1937 immunoprecipitations 1937 reciprocal experiment 1938 coprecipitation 1938 in vivo 1939 coprecipitation 1939 in vivo 1940 coimmunoprecipitates 1941 co-immunoprecipitation 1944 coprecipitated 1946 coprecipitated 1946 FLAG-tagged 1946 HA-tagged 1947 anti-HA 1947 HA-tagged 1947 immunoprecipitates 1948 antibody 1948 anti-HA 1948 GST 1948 pull-down 1948 pull-down experiments 1949 antibody 1949 anti-HA 1949 co-precipitated 1949 immunoprecipitation 1950 coimmunoprecipitation 1952 coprecipitated 1953 anti-HA 1953 precipitates 1956 Immunoprecipitation 1956 Immunoprecipitation experiments 1957 coimmunoprecipitation 1961 FLAG 1961 precipitates 1962 Flag-tagged 1964 Coimmunoprecipitation 1965 Co-immunoprecipitation 1966 anti-Flag 1966 Flag-tagged 1966 immunoprecipitation 1966 reciprocal experiment 1966 Western blotting 1967 antibodies 1967 antibodies against 1967 anti-Flag 1967 coprecipitated 1967 Flag-tagged 1967 immunoprecipitating 1968 antibodies 1968 anti-Flag 1968 Flag-tagged 1968 immunoprecipitation 1968 immunoprecipitations 1968 Western blots 1969 HA-tagged 1970 coimmunoprecipitation 1970 coimmunoprecipitation experiments 1971 coimmunoprecipitate 1973 coimmunoprecipitation 1973 coimmunoprecipitation experiments 1974 antibody 1975 cotransfection 1975 immunoprecipitation 1975 transient cotransfection 1976 antibodies 1976 antibodies against 1976 anti-Flag 1976 immunoblotting 1976 immunoprecipitates 1977 coimmunoprecipitates 1978 coimmunoprecipitates 1979 coprecipitated 1979 immunoprecipitation 1982 coimmunoprecipitation 1982 coimmunoprecipitation assays 1983 antibody 1983 anti-Flag 1983 Flag-tagged 1984 antibodies 1984 antibody 1984 anti-FLAG 1984 anti-FLAG antibody 1984 FLAG 1986 Mass spectrometry 1986 Mass spectrometry analysis 1988 immunoblotting 1991 co-immunoprecipitate 1993 coimmunoprecipitation 1993 green fluorescent protein 1993 immunoprecipitation 1993 immunoprecipitation assay 1993 in vivo 1994 antibodies 1994 antibodies against 1994 coimmunoprecipitation 1994 Flag-tagged 1994 immunoprecipitates 1995 Immunoblot 1995 Immunoblot analysis 1997 immunoprecipitate 1998 immunoprecipitate 1999 coprecipitated 1999 HA-tagged 2000 coimmunoprecipitation 2000 coimmunoprecipitation experiments 2006 antibody 2006 immunoprecipitation 2007 antibody 2007 anti-Flag 2007 Flag-tagged 2007 immunoprecipitation 2009 antibody 2009 FLAG 2010 coimmunoprecipitation 2011 coimmunoprecipitation 2012 antibodies 2012 FLAG 2012 FLAG-tagged 2013 coimmunoprecipitate 2013 FLAG-tagged 2013 HA-tagged 2014 coimmunoprecipitate 2014 FLAG-tagged 2014 HA-tagged 2017 Pull-down 2017 Pull-down assays 2018 immunoprecipitates 2019 Coimmunoprecipitation 2019 Co-IP 2019 coprecipitated 2020 Co-IP 2021 Co-IP 2022 coprecipitated 2023 coimmunoprecipitate 2024 Co-IP 2029 copurify 2035 coimmunoprecipitate 2036 Flag-tagged 2041 coimmunoprecipitation 2042 antibodies 2042 anti-GFP 2042 GFP 2042 immunoprecipitation 2044 coprecipitated 2045 mass-spectrometry 2045 mass-spectrometry analysis 2045 SDS-PAGE 2046 antibodies 2046 antibodies against 2046 coprecipitation 2046 staining 2046 western blot 2046 western blot analysis 2049 FLAG-tagged 2049 immunoprecipitates 2049 Western blot 2051 antibodies 2051 antibody 2067 two-hybrid 2067 two-hybrid analysis 2068 CD spectra 2070 CD spectra 2071 CD spectra 2072 circular dichroism 2073 titrations 2074 fluorescence 2074 spectroscopy 2076 fluorescence 2076 fluorescence data 2076 fluorescence titration 2076 fluorescence titration method 2076 Kd values 2076 titration 2076 titration method 2078 in vitro 2078 ThioGlo1 assay 2079 fluorescence 2079 fluorescence assay 2080 fluorescence 2080 intrinsic fluorescence 2081 fluorescence 2081 titration 2082 fluorescence 2082 titration 2083 fluorescence 2083 intrinsic fluorescence 2083 quantified binding 2084 fluorescence 2084 intrinsic fluorescence 2084 quantified binding 2086 Fluorescence 2087 Fluorescence 2088 fluorescence 2091 two-hybrid 2091 two-hybrid assay 2091 yeast two-hybrid 2091 yeast two-hybrid assay 2094 two-hybrid 2094 two-hybrid system 2099 bait 2099 two-hybrid 2099 two-hybrid screen 2099 yeast two-hybrid 2099 yeast two-hybrid screen 2100 two-hybrid 2100 two-hybrid system 2100 yeast two-hybrid 2100 yeast two-hybrid system 2102 LacZ reporter 2102 reporter genes 2104 Co-immunoprecipitation 2105 cDNA library 2108 two-hybrid 2108 two-hybrid system 2108 yeast two-hybrid 2108 yeast two-hybrid system 2109 Sequencing 2109 Sequencing analysis 2111 Sequencing 2114 reporter gene 2116 fusion protein 2116 two-hybrid 2116 two-hybrid experiments 2121 in vivo 2124 Sequence analysis 2131 domain fusion 2135 antibodies 2135 Coimmunoprecipitations 2141 coexpression 2142 two-hybrid 2142 two-hybrid assay 2142 Yeast two-hybrid 2142 Yeast two-hybrid assay 2143 fusion protein 2143 GST 2143 GST fusion 2143 pull-down 2143 pull-down assays 2143 two-hybrid 2143 yeast two-hybrid 2144 fusion protein 2144 GST 2144 GST fusion 2144 pull-down 2144 pull-down assays 2144 two-hybrid 2144 yeast two-hybrid 2145 two-hybrid 2145 two-hybrid assay 2146 two-hybrid 2146 two-hybrid assays 2146 yeast two-hybrid 2146 yeast two-hybrid assays 2147 Two-hybrid 2148 cDNA library 2149 cDNA library 2157 two-hybrid 2157 two-hybrid assay 2157 yeast two-hybrid 2157 yeast two-hybrid assay 2158 two-hybrid 2158 two-hybrid system 2158 yeast two-hybrid 2158 yeast two-hybrid system 2159 in vivo 2160 in vivo 2164 two-hybrid 2164 two-hybrid assay 2165 two-hybrid 2165 two-hybrid assay 2170 bait 2173 Sequence analysis 2176 fusion proteins 2182 two-hybrid 2182 two-hybrid system 2183 cDNA library 2183 two-hybrid 2183 two-hybrid technique 2183 yeast two-hybrid 2183 yeast two-hybrid technique 2187 Sequence analysis 2187 two-hybrid 2187 two-hybrid assay 2188 immunoprecipitation 2189 bait 2189 prey 2189 two-hybrid 2189 two-hybrid assay 2189 yeast two-hybrid 2189 yeast two-hybrid assay 2191 PCR 2191 preys 2191 staining 2191 two-hybrid 2191 two-hybrid assay 2196 two-hybrid 2196 two-hybrid screening 2198 immunoprecipitation 2199 immunoprecipitation 2200 fusion protein 2205 fusion proteins 2206 fusion proteins 2208 two-hybrid 2208 two-hybrid system 2210 bait 2210 bait plasmids 2210 two-hybrid 2210 two-hybrid system 2211 two-hybrid 2211 Yeast two-hybrid 2214 two-hybrid 2214 two-hybrid system 2214 yeast two-hybrid 2214 yeast two-hybrid system 2215 staining 2215 Two-hybrid 2215 Two-hybrid analysis 2216 bait 2216 two-hybrid 2216 two-hybrid method 2217 two-hybrid 2217 two-hybrid screening 2219 mutational analysis 2219 two-hybrid 2219 two-hybrid analysis 2224 two-hybrid 2224 two-hybrid system 2224 yeast two-hybrid 2224 yeast two-hybrid system 2226 cDNA library 2226 sequenced 2229 fusion proteins 2229 two-hybrid 2229 two-hybrid assay 2229 yeast two-hybrid 2229 yeast two-hybrid assay 2232 bait 2232 sequenced 2232 two-hybrid 2232 two-hybrid library 2232 yeast two-hybrid 2234 two-hybrid 2234 two-hybrid experiment 2235 two-hybrid 2235 two-hybrid system 2235 yeast two-hybrid 2235 yeast two-hybrid system 2237 two-hybrid 2237 two-hybrid system 2237 yeast two-hybrid 2237 yeast two-hybrid system 2239 coexpression 2242 galactosidase reporter 2243 two-hybrid 2243 two-hybrid screen 2243 yeast two-hybrid 2243 yeast two-hybrid screen 2244 two-hybrid 2244 two-hybrid screen 2244 yeast two-hybrid 2244 yeast two-hybrid screen 2245 two-hybrid 2245 two-hybrid assay 2246 two-hybrid 2246 two-hybrid screen 2246 yeast two-hybrid 2246 yeast two-hybrid screen 2248 two-hybrid 2248 two-hybrid system 2248 yeast two-hybrid 2248 yeast two-hybrid system 2249 antibodies 2249 binding assays 2249 in vitro 2249 in vitro binding assays 2249 two-hybrid 2249 two-hybrid assay 2249 two-hybrid experiments 2249 yeast two-hybrid 2249 yeast two-hybrid assay 2252 bait 2252 cDNA library 2252 two-hybrid 2252 two-hybrid screening 2257 coexpression 2257 two-hybrid 2257 two-hybrid assay 2257 yeast two-hybrid 2257 yeast two-hybrid assay 2259 two-hybrid 2259 two-hybrid assays 2259 yeast two-hybrid 2259 yeast two-hybrid assays 2260 two-hybrid 2260 yeast two-hybrid 2261 bait 2261 Sequencing 2263 cDNA library 2274 bait 2274 bait vector 2274 prey 2274 preys 2276 baits 2276 LacZ reporter 2276 prey 2277 baits 2277 LacZ reporter 2277 prey 2278 two-hybrid 2278 yeast two-hybrid 2282 two-hybrid 2282 two-hybrid screen 2283 two-hybrid 2283 two-hybrid assay 2284 bait 2284 two-hybrid 2284 two-hybrid system 2284 yeast two-hybrid 2284 yeast two-hybrid system 2285 bait 2285 fusion protein 2285 two-hybrid 2285 two-hybrid screen 2288 two-hybrid 2288 two-hybrid screen 2294 bait 2294 two-hybrid 2294 two-hybrid library 2294 yeast two-hybrid 2295 bait 2295 two-hybrid 2295 two-hybrid library 2295 yeast two-hybrid 2296 GST 2296 GST pull-down 2296 GST pull-down assay 2296 pull-down 2296 pull-down assay 2297 in vivo 2297 two-hybrid 2297 two-hybrid system 2297 yeast two-hybrid 2297 yeast two-hybrid system 2299 bait 2300 bait 2300 prey 2301 in vivo 2302 bait 2302 prey 2302 two-hybrid 2302 yeast two-hybrid 2303 bait 2303 prey 2303 prey plasmids 2303 two-hybrid 2303 two-hybrid analysis 2305 cDNA library 2305 two-hybrid 2305 two-hybrid screen 2305 yeast two-hybrid 2305 yeast two-hybrid screen 2307 two-hybrid 2307 two-hybrid library 2310 chimera 2314 two-hybrid 2314 two-hybrid assay 2314 two-hybrid assays 2315 two-hybrid 2315 two-hybrid system 2316 bait 2316 cDNA library 2316 two-hybrid 2316 yeast two-hybrid 2317 two-hybrid 2317 two-hybrid system 2318 cDNA library 2319 cDNA library 2319 two-hybrid 2321 two-hybrid 2321 two-hybrid assay 2321 yeast two-hybrid 2321 yeast two-hybrid assay 2323 two-hybrid 2323 two-hybrid assay 2323 yeast two-hybrid 2323 yeast two-hybrid assay 2324 two-hybrid 2324 two-hybrid screen 2325 two-hybrid 2325 two-hybrid screen 2326 Two-hybrid 2328 Bait 2328 Two-hybrid 2330 two-hybrid 2330 two-hybrid assay 2330 yeast two-hybrid 2330 yeast two-hybrid assay 2331 bait 2331 cDNA library 2331 two-hybrid 2331 two-hybrid screening 2331 yeast two-hybrid 2332 bait 2332 cDNA library 2332 two-hybrid 2332 two-hybrid screening 2332 yeast two-hybrid 2333 two-hybrid 2333 two-hybrid system 2333 yeast two-hybrid 2333 yeast two-hybrid system 2334 yeast-two-hybrid 2335 two-hybrid 2335 two-hybrid system 2335 yeast two-hybrid 2335 yeast two-hybrid system 2336 two-hybrid 2336 two-hybrid system 2336 yeast two-hybrid 2336 yeast two-hybrid system 2338 sequence analysis 2339 sequence analysis 2340 LacZ reporter 2340 reporter genes 2342 bait 2342 two-hybrid 2342 two-hybrid screening 2342 yeast two-hybrid 2343 fusion proteins 2343 two-hybrid 2346 two-hybrid 2346 two-hybrid assay 2346 yeast two-hybrid 2346 yeast two-hybrid assay 2347 BLAST 2347 sequenced 2348 two-hybrid 2348 two-hybrid screen 2348 yeast two-hybrid 2348 yeast two-hybrid screen 2350 baits 2350 reporter gene 2350 two-hybrid 2350 two-hybrid assays 2350 yeast two-hybrid 2350 yeast two-hybrid assays 2351 cDNA library 2351 two-hybrid 2351 two-hybrid screening 2351 yeast two-hybrid 2352 bait 2352 two-hybrid 2352 two-hybrid screening 2352 yeast two-hybrid 2354 two-hybrid 2354 two-hybrid assay 2354 Yeast two-hybrid 2354 Yeast two-hybrid assay 2355 LacZ reporter 2356 LacZ reporter 2358 two-hybrid 2358 two-hybrid system 2360 two-hybrid 2360 two-hybrid screening 2360 yeast two-hybrid 2362 reporter gene 2362 two hybrid 2363 two-hybrid 2363 two-hybrid system 2365 bait 2365 two-hybrid 2365 two-hybrid screen 2365 yeast two-hybrid 2365 yeast two-hybrid screen 2368 bait 2368 cDNA library 2368 two-hybrid 2368 two-hybrid screen 2368 yeast two-hybrid 2368 yeast two-hybrid screen 2370 two-hybrid 2370 yeast two-hybrid 2371 two-hybrid 2371 two-hybrid system 2371 two-hybrid system screen 2371 yeast two-hybrid 2371 yeast two-hybrid system 2371 yeast two-hybrid system screen 2374 two-hybrid 2374 two-hybrid screening 2374 two-hybrid screening system 2374 yeast two-hybrid 2379 bait 2379 two-hybrid 2379 two-hybrid screen 2379 yeast two-hybrid 2379 yeast two-hybrid screen 2380 two-hybrid 2380 two-hybrid system 2381 bait 2381 two-hybrid 2381 two-hybrid assay 2382 bait 2382 cDNA library 2382 two-hybrid 2382 two-hybrid screening 2383 two-hybrid 2383 two-hybrid assay 2383 yeast two-hybrid 2383 yeast two-hybrid assay 2385 bait 2385 baits 2385 LacZ reporter 2385 prey 2385 preys 2385 reporter gene 2385 two-hybrid 2385 two-hybrid screens 2386 bait 2386 bait plasmid 2386 prey 2386 prey plasmids 2386 reporter genes 2387 bait-vector 2390 two-hybrid 2391 two-hybrid 2395 two-hybrid 2395 two-hybrid screen 2397 two-hybrid 2397 two-hybrid assay 2399 two-hybrid 2399 two-hybrid system 2399 yeast two-hybrid 2399 yeast two-hybrid system 2407 two-hybrid 2407 two-hybrid system 2408 two-hybrid 2408 two-hybrid assay 2408 two-hybrid assay results 2408 yeast two-hybrid 2408 yeast two-hybrid assay 2408 yeast two-hybrid assay results 2413 bait 2413 two-hybrid 2413 two-hybrid system 2415 baits 2415 cDNA library 2415 Sequencing 2419 two-hybrid 2419 yeast two-hybrid 2420 two-hybrid 2420 yeast two-hybrid 2421 two-hybrid 2421 yeast two-hybrid 2422 two-hybrid 2422 yeast two-hybrid 2423 two-hybrid 2423 yeast two-hybrid 2424 two-hybrid 2424 yeast two-hybrid 2425 bait 2425 cDNA library 2430 bait 2438 two-hybrid 2439 two-hybrid 2439 two-hybrid analysis 2440 sequence similarity 2440 two-hybrid 2440 two-hybrid screen 2440 yeast two-hybrid 2440 yeast two-hybrid screen 2441 two-hybrid 2441 Yeast two-hybrid 2442 coexpression 2442 cotransfection 2442 cotransfection experiments 2444 co-immunoprecipitation 2444 co-immunoprecipitation experiments 2444 two-hybrid 2444 two-hybrid system 2444 yeast two-hybrid 2444 yeast two-hybrid system 2445 in vivo 2446 bait 2446 two-hybrid 2446 two-hybrid screening 2446 yeast two-hybrid 2447 bait 2447 baits 2449 two-hybrid 2449 yeast two-hybrid 2452 fusion proteins 2455 bait 2455 cDNA library 2456 bait 2456 cDNA library 2460 in vivo 2461 interaction assay 2461 in vivo 2464 Two-hybrid 2467 two-hybrid 2467 two-hybrid assay 2467 yeast two-hybrid 2467 yeast two-hybrid assay 2469 two-hybrid 2469 two-hybrid assay 2469 yeast two-hybrid 2469 yeast two-hybrid assay 2470 two-hybrid 2470 two-hybrid assay 2472 two-hybrid 2472 two-hybrid system 2472 yeast two-hybrid 2472 yeast two-hybrid system 2481 bait 2483 bait 2483 bait vector 2483 cDNA library 2483 two-hybrid 2483 two-hybrid screens 2483 yeast two-hybrid 2483 yeast two-hybrid screens 2484 bait 2484 two-hybrid 2484 two-hybrid screening 2484 yeast two-hybrid 2485 mammalian two hybrid 2485 two hybrid 2486 mammalian two hybrid 2486 two hybrid 2487 two-hybrid 2487 two-hybrid assays 2487 yeast two-hybrid 2487 yeast two-hybrid assays 2488 two-hybrid 2488 two-hybrid assays 2488 yeast two-hybrid 2488 yeast two-hybrid assays 2489 cDNA library 2489 two-hybrid 2489 two-hybrid screen 2489 yeast two-hybrid 2489 yeast two-hybrid screen 2491 bait 2491 cDNA library 2491 sequence analysis 2493 fusion proteins 2493 reporter gene 2494 reporter genes 2496 cDNA library 2496 staining 2497 Two-hybrid 2497 Two-hybrid analyses 2501 two-hybrid 2501 two-hybrid screening 2501 yeast two-hybrid 2502 LacZ reporter 2504 reporter gene 2506 two-hybrid 2506 two-hybrid assays 2506 yeast two-hybrid 2506 yeast two-hybrid assays 2507 two-hybrid 2507 two-hybrid assay 2507 yeast two-hybrid 2507 yeast two-hybrid assay 2508 two-hybrid 2508 two-hybrid assay 2508 yeast two-hybrid 2508 yeast two-hybrid assay 2512 fusion protein 2513 Two hybrid 2513 Two hybrid assay 2514 bait 2514 cDNA library 2514 two-hybrid 2514 two-hybrid screen 2514 yeast two-hybrid 2514 yeast two-hybrid screen 2515 two-hybrid 2515 two-hybrid analysis 2515 yeast two-hybrid 2515 yeast two-hybrid analysis 2516 two-hybrid 2517 bait 2520 GST 2520 GST pull-down 2520 GST pull-down assay 2520 in vitro 2520 pull-down 2520 pull-down assay 2520 two-hybrid 2520 two-hybrid method 2520 yeast two-hybrid 2520 yeast two-hybrid method 2521 two-hybrid 2521 two-hybrid system 2521 yeast two-hybrid 2521 yeast two-hybrid system 2524 two-hybrid 2524 two-hybrid screening 2524 yeast two-hybrid 2525 two-hybrid 2525 two-hybrid system 2525 yeast two-hybrid 2525 yeast two-hybrid system 2526 Prey 2529 in vivo 2529 two-hybrid 2529 two-hybrid assay 2529 yeast two-hybrid 2529 yeast two-hybrid assay 2530 two-hybrid 2530 yeast two-hybrid 2531 two-hybrid 2531 yeast two-hybrid 2539 co-immunoprecipitation 2539 two-hybrid 2541 two-hybrid 2541 two-hybrid assays 2544 two-hybrid 2544 two-hybrid assay 2545 two-hybrid 2546 two-hybrid 2546 two-hybrid assay 2548 in vitro 2558 bait 2558 two-hybrid 2558 two-hybrid screening 2558 yeast two-hybrid 2561 bait 2564 bait 2565 bait 2572 reporter gene 2574 Two-hybrid 2574 Two-hybrid analysis 2575 Two-hybrid 2575 Two-hybrid experiments 2576 bait 2577 bait 2577 two-hybrid 2577 yeast two-hybrid 2578 bait 2578 two-hybrid 2578 yeast two-hybrid 2579 bait 2579 two-hybrid 2579 yeast two-hybrid 2580 prey 2588 two-hybrid 2588 two-hybrid system 2588 yeast two-hybrid 2588 yeast two-hybrid system 2589 two-hybrid 2589 two-hybrid analysis 2589 Yeast two-hybrid 2589 Yeast two-hybrid analysis 2591 BLAST 2595 bait 2595 cDNA library 2595 yeast-two-hybrid 2597 bait 2597 two-hybrid 2597 two-hybrid screen 2597 yeast two-hybrid 2597 yeast two-hybrid screen 2598 bait 2598 two-hybrid 2598 two-hybrid assay 2598 two-hybrid screen 2598 yeast two-hybrid 2598 yeast two-hybrid assay 2598 yeast two-hybrid screen 2607 two-hybrid 2607 two-hybrid assays 2607 yeast two-hybrid 2607 yeast two-hybrid assays 2617 two-hybrid 2617 two-hybrid assay 2617 yeast two-hybrid 2617 yeast two-hybrid assay 2619 two-hybrid 2619 two-hybrid assay 2619 yeast two-hybrid 2619 yeast two-hybrid assay 2620 fusion proteins 2620 interaction system 2620 mammalian two-hybrid 2620 two-hybrid 2621 two-hybrid 2621 two-hybrid screening 2621 yeast two-hybrid 2626 bait 2626 prey 2628 two-hybrid 2628 two-hybrid assay 2628 yeast two-hybrid 2628 yeast two-hybrid assay 2629 fusion proteins 2629 prey 2630 in vitro 2630 in vivo 2632 two-hybrid 2632 two-hybrid systems 2643 two-hybrid 2643 two-hybrid systems 2646 two hybrid 2650 two-hybrid 2650 two-hybrid assays 2650 Yeast two-hybrid 2650 Yeast two-hybrid assays 2653 two-hybrid 2653 two-hybrid system 2655 two-hybrid 2655 two-hybrid system 2655 yeast two-hybrid 2655 yeast two-hybrid system 2657 bait 2662 two-hybrid 2662 two-hybrid analysis 2665 two-hybrid 2665 two-hybrid assay 2665 yeast two-hybrid 2665 yeast two-hybrid assay 2668 two-hybrid 2668 two-hybrid screens 2668 yeast two-hybrid 2668 yeast two-hybrid screens 2669 protein fusions 2672 fusion protein 2672 GST 2672 GST fusion 2672 in vitro 2672 pull down 2672 pull down experiment 2672 two hybrid 2672 two hybrid assay 2672 yeast two hybrid 2672 yeast two hybrid assay 2676 in vitro 2678 bait 2678 cDNA library 2678 two-hybrid 2678 yeast two-hybrid 2680 prey 2682 bait 2682 prey 2685 bait 2685 reporter genes 2685 Sequence analyses 2685 two-hybrid 2685 two-hybrid screening 2685 two-hybrid screening system 2685 yeast two-hybrid 2687 two hybrid 2688 two-hybrid 2688 two-hybrid screen 2688 yeast two-hybrid 2688 yeast two-hybrid screen 2698 cDNA library 2698 two-hybrid 2698 two-hybrid method 2703 bait 2703 two-hybrid 2703 two-hybrid screening 2703 Yeast two-hybrid 2705 cDNA library 2705 two-hybrid 2705 two-hybrid system 2705 yeast two-hybrid 2705 yeast two-hybrid system 2706 two-hybrid 2706 yeast two-hybrid 2708 two-hybrid 2708 two-hybrid assay 2711 bait 2711 two-hybrid 2711 two-hybrid screen 2711 yeast two-hybrid 2711 yeast two-hybrid screen 2712 bait 2712 cDNA library 2712 Sequence analysis 2713 bait 2713 bait vector 2714 bait 2714 cDNA library 2714 two-hybrid 2714 two-hybrid system 2714 yeast two-hybrid 2714 yeast two-hybrid system 2718 Bait 2718 prey 2718 two-hybrid 2718 two-hybrid system 2719 bait 2719 bait plasmid 2719 prey 2719 prey plasmid 2719 two-hybrid 2719 two-hybrid library 2719 yeast genomic two-hybrid 2720 in vitro 2720 Overlay experiments 2720 pull-down 2720 two-hybrid 2720 two-hybrid assays 2721 in vitro 2721 Overlay experiments 2721 pull-down 2721 two-hybrid 2721 two-hybrid assays 2722 two-hybrid 2722 two-hybrid screen 2722 yeast two-hybrid 2722 yeast two-hybrid screen 2727 reporter genes 2731 two-hybrid 2731 two-hybrid assay 2732 cDNA library 2735 cDNA library 2736 interaction assay 2736 in vivo 2736 two-hybrid 2736 two-hybrid assay 2736 yeast two-hybrid 2736 yeast two-hybrid assay 2738 two-hybrid 2738 two-hybrid analysis 2738 Yeast two-hybrid 2738 Yeast two-hybrid analysis 2742 Mammalian two-hybrid 2742 two-hybrid 2745 baits 2746 two-hybrid 2746 two-hybrid screen 2746 yeast two-hybrid 2746 yeast two-hybrid screen 2747 prey 2747 two-hybrid 2747 two-hybrid system 2747 yeast two-hybrid 2747 yeast two-hybrid system 2748 pulldown 2748 two-hybrid 2748 yeast two-hybrid 2749 prey 2753 two-hybrid 2753 two-hybrid analysis 2753 Yeast two-hybrid 2753 Yeast two-hybrid analysis 2755 bait 2755 two-hybrid 2755 two-hybrid screen 2756 bait 2756 two-hybrid 2756 two-hybrid screen 2758 co-immunoprecipitation 2758 co-immunoprecipitation assay 2758 in vivo 2758 two-hybrid 2758 two-hybrid analysis 2758 yeast two-hybrid 2758 yeast two-hybrid analysis 2761 two-hybrid 2761 two-hybrid analysis 2761 Yeast two-hybrid 2761 Yeast two-hybrid analysis 2764 co-immunoprecipitation 2764 mammalian two-hybrid 2764 two-hybrid 2766 two-hybrid 2766 yeast two-hybrid 2768 two-hybrid 2768 two-hybrid assays 2768 yeast two-hybrid 2768 yeast two-hybrid assays 2771 bait 2771 cDNA library 2771 fusion protein 2772 bait 2772 cDNA library 2772 fusion protein 2777 Sequence analysis 2778 bait 2778 cDNA library 2780 two-hybrid 2780 two-hybrid assay 2780 yeast two-hybrid 2780 yeast two-hybrid assay 2782 two-hybrid 2782 two-hybrid analysis 2782 yeast two-hybrid 2782 yeast two-hybrid analysis 2783 bait 2783 sequenced 2783 two-hybrid 2783 two-hybrid screen 2783 yeast two-hybrid 2783 yeast two-hybrid screen 2785 fusion proteins 2788 two hybrid 2788 two hybrid system 2789 two hybrid 2789 two hybrid analysis 2790 bait 2790 two-hybrid 2790 two-hybrid screening 2790 yeast two-hybrid 2795 two-hybrid 2795 two-hybrid analysis 2795 Yeast two-hybrid 2795 Yeast two-hybrid analysis 2800 BLAST 2802 in vivo 2802 pull-down 2802 pull-down assays 2802 the Y2H system 2804 in vitro 2809 cDNA library 2809 two-hybrid 2809 two-hybrid screen 2809 yeast two-hybrid 2809 yeast two-hybrid screen 2810 bait 2810 reporter genes 2810 two-hybrid 2810 two-hybrid system 2810 yeast two-hybrid 2810 yeast two-hybrid system 2811 cDNA library 2811 reporter gene 2811 Sequencing 2812 bait 2812 cDNA library 2813 coexpression 2813 Coexpression 2814 cotransfection 2815 bait 2815 two-hybrid 2815 two-hybrid screening 2815 yeast two-hybrid 2817 two-hybrid 2817 two-hybrid assay 2817 yeast two-hybrid 2817 yeast two-hybrid assay 2818 bait 2818 cDNA library 2818 prey 2820 two-hybrid 2820 two-hybrid experiment 2820 yeast two-hybrid 2820 yeast two-hybrid experiment 2821 bait 2821 two hybrid 2821 yeast two hybrid 2822 two-hybrid 2822 two-hybrid assay 2822 yeast two-hybrid 2822 yeast two-hybrid assay 2823 bait 2823 cDNA library 2823 two-hybrid 2823 two-hybrid screen 2823 yeast two-hybrid 2823 yeast two-hybrid screen 2824 two-hybrid 2824 two-hybrid assay 2824 yeast two-hybrid 2824 yeast two-hybrid assay 2825 two-hybrid 2825 two-hybrid assays 2827 interaction assays 2827 reporter genes 2827 two hybrid 2827 two hybrid interaction assays 2831 chimera 2831 reporter-gene 2832 bait 2832 Sequencing 2832 two-hybrid 2832 two-hybrid screen 2832 yeast two-hybrid 2832 yeast two-hybrid screen 2834 two-hybrid 2835 domain fusion 2835 fusion protein 2835 two-hybrid 2835 two-hybrid system 2836 bait 2837 bait 2838 bait 2838 cDNA library 2838 two-hybrid 2838 yeast two-hybrid 2841 Two-hybrid 2841 Two-hybrid analysis 2844 bait 2844 cDNA library 2844 two-hybrid 2844 two-hybrid screen 2844 yeast two-hybrid 2844 yeast two-hybrid screen 2845 bait 2845 bait vector 2847 two-hybrid 2847 two-hybrid screen 2849 reconstitution analysis 2850 bait 2850 cDNA library 2851 interaction analysis 2853 two-hybrid 2853 two-hybrid analysis 2853 yeast two-hybrid 2853 yeast two-hybrid analysis 2855 bait 2855 prey 2857 bait 2857 cDNA library 2857 two-hybrid 2857 two-hybrid screen 2857 yeast two-hybrid 2857 yeast two-hybrid screen 2859 two-hybrid 2859 two-hybrid assays 2859 yeast two-hybrid 2859 yeast two-hybrid assays 2860 cDNA library 2861 bait 2861 prey 2861 two-hybrid 2861 two-hybrid assay 2861 yeast two-hybrid 2861 yeast two-hybrid assay 2864 two-hybrid 2864 two-hybrid screening 2864 yeast two-hybrid 2866 fusion protein 2866 two-hybrid 2866 two-hybrid system 2866 yeast two-hybrid 2866 yeast two-hybrid system 2867 two-hybrid 2867 two-hybrid assay 2867 yeast two-hybrid 2867 yeast two-hybrid assay 2868 two-hybrid 2868 two-hybrid system 2868 yeast two-hybrid 2868 yeast two-hybrid system 2869 bait 2869 cDNA library 2869 Sequence analysis 2871 two-hybrid 2871 Yeast two-hybrid 2872 co-immunoprecipitation 2872 two-hybrid 2872 two-hybrid experiments 2872 yeast two-hybrid 2872 yeast two-hybrid experiments 2873 two-hybrid 2873 two-hybrid screening 2873 yeast two-hybrid 2874 bait 2874 baits 2874 cDNA library 2874 Sequence analysis 2875 Two-hybrid 2875 Two-hybrid analysis 2877 in vivo 2878 two-hybrid 2878 two-hybrid system 2878 yeast two-hybrid 2878 yeast two-hybrid system 2879 two-hybrid 2879 two-hybrid analysis 2879 yeast two-hybrid 2879 yeast two-hybrid analysis 2880 bait 2880 cDNA library 2880 two-hybrid 2880 two-hybrid screen 2880 yeast two-hybrid 2880 yeast two-hybrid screen 2883 activity assays 2883 bait 2885 interaction assay 2885 two-hybrid 2885 two-hybrid interaction assay 2885 yeast two-hybrid 2886 interaction assay 2886 two-hybrid 2886 two-hybrid interaction assay 2886 yeast two-hybrid 2887 two-hybrid 2887 two-hybrid screen 2887 yeast two-hybrid 2887 yeast two-hybrid screen 2903 cDNA library 2903 Sequence analysis 2903 two-hybrid 2903 two-hybrid assay 2903 yeast two-hybrid 2903 yeast two-hybrid assay 2904 bait 2904 two-hybrid 2904 two-hybrid method 2905 bait 2906 bait 2906 two-hybrid 2906 two-hybrid assay 2914 bait 2914 cDNA library 2914 two-hybrid 2914 two-hybrid analysis 2914 yeast two-hybrid 2914 yeast two-hybrid analysis 2915 Two-hybrid 2917 bait 2917 cDNA library 2917 two-hybrid 2917 two-hybrid screen 2917 yeast two-hybrid 2917 yeast two-hybrid screen 2918 two hybrid 2918 two hybrid assay 2918 Yeast two hybrid 2918 Yeast two hybrid assay 2920 bait 2920 two-hybrid 2920 two-hybrid screen 2920 yeast two-hybrid 2920 yeast two-hybrid screen 2921 two-hybrid 2921 two-hybrid analyses 2921 yeast two-hybrid 2921 yeast two-hybrid analyses 2923 baits 2923 Baits 2923 BLAST 2923 two-hybrid 2923 two-hybrid method 2923 yeast two-hybrid 2923 yeast two-hybrid method 2924 sequenced 2924 two-hybrid 2924 two-hybrid system 2924 yeast two-hybrid 2924 yeast two-hybrid system 2925 ESR 2926 two-hybrid 2926 two-hybrid screen 2926 yeast two-hybrid 2926 yeast two-hybrid screen 2928 bait 2928 yeast-two-hybrid 2929 yeast-two-hybrid 2931 two hybrid 2931 two hybrid system 2932 bridge assay 2932 fusion proteins 2936 two-hybrid 2936 two-hybrid assay 2936 yeast two-hybrid 2936 yeast two-hybrid assay 2939 two-hybrid 2939 two-hybrid assay 2939 Yeast two-hybrid 2939 Yeast two-hybrid assay 2940 two-hybrid 2940 two-hybrid assay 2940 Yeast two-hybrid 2940 Yeast two-hybrid assay 2941 bait 2941 baits 2941 bait vector 2941 prey 2941 prey plasmids 2946 reporter gene 2948 bait 2948 prey 2948 reporter genes 2949 two-hybrid 2949 yeast two-hybrid 2950 two-hybrid 2950 two-hybrid assays 2950 yeast two-hybrid 2950 yeast two-hybrid assays 2951 two-hybrid 2951 two-hybrid assays 2951 yeast two-hybrid 2951 yeast two-hybrid assays 2952 cDNA library 2952 two-hybrid 2952 two-hybrid screening 2952 yeast two-hybrid 2953 two-hybrid 2953 two-hybrid analysis 2954 two-hybrid 2954 two-hybrid analysis 2954 yeast two-hybrid 2954 yeast two-hybrid analysis 2955 two-hybrid 2955 two-hybrid screening 2955 Yeast two-hybrid 2956 interaction analysis 2956 two-hybrid 2956 two-hybrid interaction analysis 2956 yeast two-hybrid 2959 two-hybrid 2959 two-hybrid screens 2959 yeast two-hybrid 2959 yeast two-hybrid screens 2960 two-hybrid 2960 two-hybrid system 2962 bait 2962 cDNA library 2963 two-hybrid 2963 two-hybrid screening 2963 Yeast two-hybrid 2964 prey 2967 two-hybrid 2967 two-hybrid assay 2967 yeast two-hybrid 2967 yeast two-hybrid assay 2968 bait 2968 cDNA library 2970 bait 2970 prey 2971 two hybrid 2971 two hybrid system 2971 yeast two hybrid 2971 yeast two hybrid system 2972 bait 2972 two-hybrid 2972 two-hybrid screening 2972 yeast two-hybrid 2973 Coexpression 2976 two-hybrid 2976 two-hybrid assays 2976 Yeast two-hybrid 2976 Yeast two-hybrid assays 2981 prey 2981 two-hybrid 2981 two-hybrid assays 2981 yeast two-hybrid 2981 yeast two-hybrid assays 2982 two-hybrid 2982 two-hybrid assays 2982 yeast two-hybrid 2982 yeast two-hybrid assays 2983 two-hybrid 2983 two-hybrid screen 2983 yeast two-hybrid 2983 yeast two-hybrid screen 2984 bait 2984 two-hybrid 2984 two-hybrid screens 2986 bait 2986 baits 2986 prey 2986 prey plasmids 2986 reporter gene 2987 two-hybrid 2987 two-hybrid assays 2988 two-hybrid 2988 two-hybrid assays 2990 Sequencing 2994 bait 2994 cDNA library 2994 sequenced 2995 two-hybrid 2995 two-hybrid assay 2995 yeast two-hybrid 2995 yeast two-hybrid assay 2997 two hybrid 2997 two hybrid assays 2997 yeast two hybrid 2997 yeast two hybrid assays 3000 bait 3000 cDNA library 3000 two-hybrid 3000 yeast two-hybrid 3007 two-hybrid 3007 two-hybrid assays 3007 yeast two-hybrid 3007 yeast two-hybrid assays 3008 bait 3008 cDNA library 3008 Sequence analysis 3008 two-hybrid 3008 two-hybrid technique 3008 yeast two-hybrid 3008 yeast two-hybrid technique 3012 in vitro 3013 cDNA library 3013 two-hybrid 3013 two-hybrid system 3013 yeast two-hybrid 3013 yeast two-hybrid system 3014 bait 3014 bait plasmid 3014 prey 3014 prey plasmid 3015 bait 3015 cDNA library 3015 two-hybrid 3015 yeast two-hybrid 3017 sequence similarity 3019 two-hybrid 3019 two-hybrid results 3019 yeast two-hybrid 3019 yeast two-hybrid results 3023 two-hybrid 3023 yeast two-hybrid 3024 preys 3024 sequenced 3024 two-hybrid 3024 yeast two-hybrid 3025 two-hybrid 3025 two-hybrid assays 3025 yeast two-hybrid 3025 yeast two-hybrid assays 3027 two-hybrid 3027 two-hybrid assays 3027 yeast two-hybrid 3027 yeast two-hybrid assays 3032 bait 3032 prey 3032 two-hybrid 3032 two-hybrid analyses 3032 yeast two-hybrid 3032 yeast two-hybrid analyses 3037 two-hybrid 3037 two-hybrid screening 3037 yeast two-hybrid 3038 bait 3038 cDNA library 3038 two-hybrid 3038 two-hybrid system 3038 yeast two-hybrid 3038 yeast two-hybrid system 3039 two-hybrid 3039 two-hybrid system 3039 yeast two-hybrid 3039 yeast two-hybrid system 3047 two-hybrid 3047 two-hybrid screen 3047 yeast two-hybrid 3047 yeast two-hybrid screen 3052 bait 3052 two-hybrid 3052 two-hybrid screening 3052 yeast two-hybrid 3054 two-hybrid 3054 two-hybrid system 3054 yeast two-hybrid 3054 yeast two-hybrid system 3055 reporter gene 3057 two-hybrid 3057 two-hybrid system 3057 yeast two-hybrid 3057 yeast two-hybrid system 3058 bait 3058 two-hybrid 3058 two-hybrid screening 3058 yeast two-hybrid 3059 two-hybrid 3059 two-hybrid system 3059 yeast two-hybrid 3059 yeast two-hybrid system 3060 cDNA library 3060 two-hybrid 3060 two-hybrid screening 3060 yeast two-hybrid 3062 two-hybrid 3062 two-hybrid screening 3062 yeast two-hybrid 3063 two-hybrid 3063 two-hybrid screening 3063 yeast two-hybrid 3064 two-hybrid 3064 two-hybrid assay 3064 yeast two-hybrid 3064 yeast two-hybrid assay 3066 bait 3066 sequenced 3067 bait 3067 fusion proteins 3067 two-hybrid 3067 yeast two-hybrid 3069 bait 3069 cDNA library 3069 two-hybrid 3069 two-hybrid method 3069 yeast two-hybrid 3069 yeast two-hybrid method 3070 bait 3070 cDNA library 3070 two-hybrid 3070 two-hybrid method 3070 yeast two-hybrid 3070 yeast two-hybrid method 3071 bait 3071 cDNA library 3071 cDNA library screen 3071 two hybrid 3071 yeast two hybrid 3076 two-hybrid 3076 two-hybrid assay 3076 yeast two-hybrid 3076 yeast two-hybrid assay 3077 bait 3077 two-hybrid 3077 two-hybrid screen 3077 yeast two-hybrid 3077 yeast two-hybrid screen 3078 bait 3078 prey 3078 reporter gene 3078 two-hybrid 3078 two-hybrid assay 3078 yeast two-hybrid 3078 yeast two-hybrid assay 3080 two-hybrid 3080 two-hybrid assays 3080 yeast two-hybrid 3080 yeast two-hybrid assays 3081 bait 3081 preys 3081 two-hybrid 3081 two-hybrid assay 3081 yeast two-hybrid 3081 yeast two-hybrid assay 3082 bait 3082 prey 3086 prey 3086 two-hybrid 3086 two-hybrid assay 3089 cDNA library 3089 two-hybrid 3089 two-hybrid system 3089 yeast two-hybrid 3089 yeast two-hybrid system 3091 two-hybrid 3091 two-hybrid system 3093 bait 3093 cDNA library 3093 two-hybrid 3093 two-hybrid screen 3093 yeast two-hybrid 3093 yeast two-hybrid screen 3096 bait 3096 cDNA library 3106 in vitro 3108 bait 3110 bait 3115 baits 3115 cDNA library 3118 two-hybrid 3118 two-hybrid library 3118 yeast two-hybrid 3123 bait 3123 prey 3123 two-hybrid 3123 two-hybrid assay 3123 two-hybrid screen 3123 yeast two-hybrid 3123 yeast two-hybrid assay 3123 yeast two-hybrid screen 3129 LacZ reporter 3129 LacZ reporter analysis 3129 reporter analysis 3131 bait 3131 prey 3131 two-hybrid 3131 yeast two-hybrid 3132 bait 3132 two-hybrid 3132 two-hybrid library 3132 two-hybrid library screen 3132 yeast two-hybrid 3133 two-hybrid 3133 two-hybrid assays 3133 yeast two-hybrid 3133 yeast two-hybrid assays 3135 bait 3135 two-hybrid 3135 two-hybrid screen 3135 yeast two-hybrid 3135 yeast two-hybrid screen 3136 reporter genes 3136 two-hybrid 3136 two-hybrid assays 3136 yeast two-hybrid 3136 yeast two-hybrid assays 3137 two-hybrid 3137 two-hybrid assay 3137 yeast two-hybrid 3137 yeast two-hybrid assay 3138 reporter genes 3139 reporter genes 3141 bait 3141 two-hybrid 3141 two-hybrid system 3141 yeast two-hybrid 3141 yeast two-hybrid system 3142 cDNA library 3145 co-localize 3146 bait 3146 bait vector 3147 bait 3147 prey 3149 bait 3149 prey 3149 the Y2H screen 3149 two-hybrid 3149 two-hybrid assay 3149 Yeast two-hybrid 3149 Yeast two-hybrid assay 3150 bait 3150 cDNA library 3150 cDNA library screen 3150 two-hybrid 3150 yeast two-hybrid 3151 two-hybrid 3151 two-hybrid system 3151 yeast two-hybrid 3151 yeast two-hybrid system 3152 bait 3152 fusion protein 3152 prey 3154 two-hybrid 3154 yeast two-hybrid 3158 two hybrid 3158 two hybrid library 3158 yeast two hybrid 3161 bait 3161 two-hybrid 3161 two-hybrid screens 3161 yeast two-hybrid 3161 yeast two-hybrid screens 3162 BLAST 3162 co-immunoprecipitation 3164 two-hybrid 3164 two-hybrid system 3164 yeast two-hybrid 3164 yeast two-hybrid system 3168 bait 3168 two-hybrid 3168 two-hybrid screens 3168 yeast two-hybrid 3168 yeast two-hybrid screens 3169 bait 3169 cDNA library 3172 two-hybrid 3172 two-hybrid analysis 3172 Yeast two-hybrid 3172 Yeast two-hybrid analysis 3177 two-hybrid 3177 two-hybrid assay 3177 Yeast two-hybrid 3177 Yeast two-hybrid assay 3179 GST 3180 in vivo 3180 two-hybrid 3181 two-hybrid 3181 two-hybrid screen 3181 yeast two-hybrid 3181 yeast two-hybrid screen 3184 two-hybrid 3184 two-hybrid analysis 3185 two-hybrid 3185 two-hybrid system 3186 two-hybrid 3186 two-hybrid system 3195 two-hybrid 3195 two-hybrid screen 3196 two-hybrid 3197 fusion protein 3197 two-hybrid 3197 two-hybrid assay 3197 yeast two-hybrid 3197 yeast two-hybrid assay 3198 domain fusions 3199 in vitro 3199 two-hybrid 3199 two-hybrid assay 3199 yeast two-hybrid 3199 yeast two-hybrid assay 3204 two-hybrid 3204 two-hybrid system 3204 yeast two-hybrid 3204 yeast two-hybrid system 3206 two hybrid 3206 two hybrid system 3208 fusion protein 3211 cDNA library 3211 two hybrid 3212 two-hybrid 3213 fusion protein 3214 two-hybrid 3214 two-hybrid assay 3215 bait 3215 two-hybrid 3215 two-hybrid screen 3216 in vivo 3216 Two-hybrid 3216 Two-hybrid analyses 3217 bait 3217 two-hybrid 3217 two-hybrid system 3217 yeast two-hybrid 3217 yeast two-hybrid system 3219 indirect assay 3219 reporter gene 3219 two-hybrid 3219 two-hybrid system 3225 two-hybrid 3225 two-hybrid screen 3225 yeast two-hybrid 3225 yeast two-hybrid screen 3226 chimera 3227 domain fusion 3227 domain fusions 3229 localizes 3233 bait 3233 cDNA library 3233 two-hybrid 3233 two-hybrid screen 3236 two-hybrid 3236 two-hybrid system 3236 yeast two-hybrid 3236 yeast two-hybrid system 3237 two-hybrid 3237 two-hybrid assay 3239 interaction system 3239 two hybrid 3239 two hybrid interaction system 3240 fusion protein 3241 fusion protein 3242 coexpression 3243 two-hybrid 3243 yeast two-hybrid 3245 two-hybrid 3246 two-hybrid 3246 two-hybrid system 3246 yeast two-hybrid 3246 yeast two-hybrid system 3247 two-hybrid 3247 two-hybrid assay 3247 Yeast two-hybrid 3247 Yeast two-hybrid assay 3249 two-hybrid 3249 two-hybrid screen 3249 yeast two-hybrid 3249 yeast two-hybrid screen 3251 staining 3251 two-hybrid 3251 two-hybrid system 3251 yeast two-hybrid 3251 yeast two-hybrid system 3253 fusion proteins 3254 bait 3257 two-hybrid 3257 two-hybrid system 3257 yeast two-hybrid 3257 yeast two-hybrid system 3259 in vivo 3260 two-hybrid 3260 two-hybrid screen 3260 yeast two-hybrid 3260 yeast two-hybrid screen 3261 bait 3262 localize 3262 two-hybrid 3262 two-hybrid assay 3262 yeast two-hybrid 3262 yeast two-hybrid assay 3264 baits 3264 two-hybrid 3267 in vivo 3267 two-hybrid 3267 two-hybrid system 3267 yeast two-hybrid 3267 yeast two-hybrid system 3270 two hybrid 3270 two hybrid system 3270 yeast two hybrid 3270 yeast two hybrid system 3276 two-hybrid 3276 two-hybrid assay 3277 bait 3278 two-hybrid 3278 two-hybrid assay 3278 yeast two-hybrid 3278 yeast two-hybrid assay 3280 two-hybrid 3280 two-hybrid assay 3280 yeast two-hybrid 3280 yeast two-hybrid assay 3283 two-hybrid 3283 two-hybrid system 3283 yeast two-hybrid 3283 yeast two-hybrid system 3285 two-hybrid 3285 two-hybrid analysis 3285 yeast two-hybrid 3285 yeast two-hybrid analysis 3286 two-hybrid 3286 two-hybrid analyses 3286 yeast two-hybrid 3286 yeast two-hybrid analyses 3287 two-hybrid 3287 two-hybrid assays 3287 yeast two-hybrid 3287 yeast two-hybrid assays 3288 bait 3288 prey 3289 GST 3289 GST pull-down 3289 in vitro 3289 pull-down 3289 yeast-two-hybrid 3290 two-hybrid 3290 yeast two-hybrid 3292 cDNA library 3292 two-hybrid 3292 two-hybrid screening 3292 yeast two-hybrid 3293 two-hybrid 3293 two-hybrid screen 3294 two-hybrid 3294 two-hybrid assays 3294 yeast two-hybrid 3294 yeast two-hybrid assays 3296 two-hybrid 3296 two-hybrid assays 3296 Yeast two-hybrid 3296 Yeast two-hybrid assays 3299 two-hybrid 3299 two-hybrid system 3299 yeast two-hybrid 3299 yeast two-hybrid system 3300 two-hybrid 3300 two-hybrid assays 3300 Yeast two-hybrid 3300 Yeast two-hybrid assays 3301 two-hybrid 3301 two-hybrid assays 3301 Yeast two-hybrid 3301 Yeast two-hybrid assays 3303 two-hybrid 3303 two-hybrid system 3303 yeast two-hybrid 3303 yeast two-hybrid system 3305 Two-hybrid 3305 Two-hybrid analysis 3306 immunoprecipitates 3307 in vivo 3308 antibody 3309 antibody 3309 Coprecipitating 3309 immunoprecipitation 3311 coprecipitation 3311 coprecipitation experiments 3319 coimmunoprecipitation 3319 in vitro 3322 GST 3323 GST 3324 GST 3325 two-hybrid 3325 two-hybrid analysis 3325 two-hybrid method 3328 co-sediment 3329 co-sediment 3330 chromatography 3330 gel filtration 3330 gel filtration chromatography 3331 glycerol velocity gradients 3332 cosedimentation 3334 antibodies 3339 binding assay 3339 in vitro 3339 in vitro binding assay 3340 SDS gels 3342 microsequencing 3342 microsequencing analysis 3343 purification 3352 in vivo 3352 Western-blot 3352 Western-blot analysis 3355 analytical ultracentrifuge 3355 in vitro 3355 velocity sedimentation 3357 density gradient centrifugation 3357 fractionation 3357 Subcellular fractionation 3357 sucrose density gradient 3359 sedimentation analysis 3359 Velocity sedimentation 3359 Velocity sedimentation analysis 3360 sedimentation analysis 3360 Velocity sedimentation 3360 Velocity sedimentation analysis 3361 coprecipitation 3364 Purification 3366 analytical ultracentrifugation 3369 sucrose gradient 3369 sucrose gradient centrifugation 3373 N-terminal sequencing 3373 SDS/PAGE 3374 sucrose gradients 3377 SDS-PAGE 3377 Western blot 3377 Western blot analysis 3378 Sucrose gradient 3380 Western blotting 3387 sedimentation experiments 3388 Cosediment 3389 in vivo 3389 sucrose density gradient 3390 Colocalization 3395 biotinylated 3396 biotinylated 3400 in situ 3400 SDS-PAGE 3401 antibody 3401 immunoprecipitate 3403 fingerprinting 3403 Peptide mass fingerprinting 3407 antibodies 3407 Antibody 3407 immunoprecipitations 3407 western-blot 3409 antibody 3409 SDS-PAGE 3410 cross-linking experiments 3411 in vitro 3412 immunoprecipitates 3414 electrophoresis 3414 gel electrophoresis 3414 SDS gel 3416 antibodies 3416 antibodies against 3416 immunoprecipitating 3416 Western blots 3417 co-purified 3417 His6-tag 3424 electrophoresis 3424 staining 3427 protein arrays 3428 colocalize 3429 colocalizes 3431 antibodies 3431 Electron microscopy 3431 labelling 3431 microscopy 3433 Colocalization 3433 electron microscopy 3433 microscopy 3434 Electron microscopy 3434 Electron microscopy analysis 3434 microscopy 3434 microscopy analysis 3435 Electron microscopy 3435 Electron microscopy analysis 3435 immunogold labeling 3435 microscopy 3435 microscopy analysis 3436 immobilized 3436 sensor chip 3438 co-localization 3438 electron micrograph 3438 immunogold labeling 3438 micrograph 3439 antibodies 3439 immunohistochemistry 3440 antibodies 3440 antibodies against 3440 colocalization 3440 immunogold labeling 3441 Electron micrographs 3441 micrographs 3447 Micrograph 3447 negatively stained 3448 Immunoelectron microscopy 3448 microscopy 3449 Immunofluorescence 3449 Immunofluorescence analysis 3450 electron microscopy 3450 microscopy 3452 SDS-PAGE 3453 GST 3453 GST fusions 3454 Far Western analysis 3454 Western analysis 3455 overlay analysis 3457 Autoradiography 3459 western blot 3462 biotinylated 3463 antibody 3463 anti-FLAG 3463 FLAG 3463 fusion protein 3463 GST 3463 GST fusion 3463 SDS-PAGE 3463 Western blotting 3466 biotinylated 3466 overlay experiments 3471 overlay assays 3475 biotinylated 3476 biotinylated 3479 GST 3480 antiserum 3481 CaM overlay assay 3481 overlay assay 3483 immunoprecipitates 3484 overlay experiments 3485 bait 3485 prey 3486 Overlay assay 3488 Western blots 3490 antibodies 3490 fusion protein 3490 in vitro 3490 SDS-PAGE 3492 in vitro 3492 in vivo 3494 fusion proteins 3494 overlay assay 3504 overlay analysis 3505 binding assays 3505 filter binding 3505 filter binding assays 3508 immobilized 3511 overlay assay 3512 Overlay experiments 3513 Overlay experiments 3515 overlay screen 3516 MALDI-TOF 3516 SDS-PAGE 3517 overlay experiments 3518 biotinylated 3522 binding assays 3522 filter binding 3522 filter binding assays 3529 binding assay 3529 filter binding 3529 filter binding assay 3530 FLAG-tagged 3532 colocalization 3532 colocalization analyses 3532 localizes 3533 colocalization 3533 immunofluorescence 3533 immunofluorescence microscopy 3533 immunofluorescence microscopy analysis 3533 microscopy 3533 microscopy analysis 3534 fluorescence 3536 fluorescence 3545 fluorescence 3546 fluorescence 3548 fluorescence 3557 fluorescence 3559 flow cytometry 3559 fluorescence 3560 antibodies 3560 antibodies against 3560 flow cytometry 3562 anti-IgA FITC 3562 FITC 3562 fusion protein 3562 staining 3563 anti-IgA FITC 3563 FITC 3563 fusion protein 3563 fusion proteins 3563 staining 3564 flow cytometry 3565 fusion protein 3567 FRET 3568 fluorescence 3568 Fluorescence 3568 protein fusions 3570 in vivo 3571 FRET 3574 FRET 3575 FRET 3575 FRET analysis 3576 FRET 3576 FRET results 3577 FRET 3578 FRET 3579 FRET 3580 FRET 3581 FRET 3584 FRET 3585 FRET 3585 FRET analysis 3588 coexpression 3588 fluorescence 3591 FRET 3591 in vivo 3592 fluorescence 3592 FRET 3592 fusion proteins 3593 FRET 3593 FRET analysis 3594 FRET 3594 FRET analysis 3595 fluorescence 3595 FRET 3596 fluorescence 3596 FRET 3596 FRET analysis 3596 in vivo 3597 FRET 3597 inference 3597 in vivo 3598 FRET 3599 FRET 3600 FRET 3603 FRET 3603 in vivo 3604 Colocalizes 3605 co-localization 3605 fusion proteins 3606 fluorescence 3606 FRET 3606 FRET technique 3609 FRET 3610 FRET 3612 Flow cytometry 3613 FRET 3614 ITC 3614 titration 3615 ITC 3616 ITC 3623 ITC 3624 Isothermal titration calorimetry 3624 ITC 3624 titration 3626 ITC 3627 isothermal titration calorimetry 3627 titration 3630 titration 3635 isothermal titration calorimetry 3635 ITC 3635 titration 3637 GST 3637 titrations 3639 laser light scattering 3639 light scattering 3639 quasi-elastic laser light scattering 3640 in vivo 3641 crystal 3641 crystal structure 3642 light-scattering 3643 fusion protein 3643 in vitro 3643 light-scattering 3645 in vitro 3645 mass spectrometry 3647 mass spectrometry 3650 electrophoresis 3651 gel filtration 3651 gel filtration analysis 3652 Gel filtration 3652 Gel filtration analysis 3653 in vitro 3655 Mass spectrometry 3656 Western blot 3656 Western blot results 3659 gel filtration 3661 antibodies 3661 chromatography 3661 elution 3661 elutions 3661 gel filtration 3661 gel filtration chromatography 3661 Western blot 3661 Western blot analysis 3663 gel filtration 3664 chromatography 3664 gel filtration 3664 gel filtration chromatography 3665 Gel filtration 3665 Gel filtration analysis 3667 gel filtration 3669 gel-filtration 3669 HA-tagged 3671 gel filtration 3671 immunoblots 3674 antibodies 3674 anti-HA 3674 Immunoblotting 3674 in vivo 3676 antibodies 3676 chromatography 3676 size exclusion chromatography 3676 Western blotting 3681 Gel-Filtration 3682 Gel filtration 3683 gel filtration 3684 Gel filtration 3686 chromatography 3686 gel filtration 3686 gel filtration experiments 3686 liquid chromatography 3687 gel-filtration 3689 antibodies 3689 antibodies against 3689 gel filtration 3689 SDS-PAGE 3690 gel filtration 3690 in vivo 3692 comigrated 3692 fractionation 3692 nocodazole 3693 gel filtration 3693 gel filtration experiments 3695 elution 3695 gel filtration 3695 gel filtration HPLC 3695 HPLC 3701 comigrated 3701 gel-filtration 3701 gel-filtration analysis 3701 pull-down 3701 pull-down results 3704 elution 3704 gel filtration 3709 chromatography 3709 gel filtration 3709 gel filtration chromatography 3712 chromatography 3712 electrophoresis 3712 elution 3712 gel electrophoresis 3712 gel filtration 3712 gel filtration chromatography 3713 In vitro 3714 chromatography 3714 gel filtration 3714 gel filtration chromatography 3717 gel filtration 3718 gel filtration 3721 purification 3723 Gel filtration 3724 chromatography 3724 size exclusion chromatography 3725 chromatography 3725 size exclusion chromatography 3727 antibodies 3728 purification 3728 Purification 3729 electrophoresis 3729 gel electrophoresis 3729 SDS gel 3729 Western blot 3729 Western blot analysis 3730 Analytical gel filtration 3730 gel filtration 3731 analytical gel filtration 3731 gel filtration 3731 proteolysis experiments 3731 sequence analyses 3733 elution 3736 SDS gel 3738 Gel filtration 3740 coexpression 3740 SDS gels 3742 gel filtration 3743 copurified 3744 copurified 3744 purification 3745 copurified 3745 purification 3746 copurified 3746 purification 3747 copurified 3748 coexpression 3748 purification 3749 Gel filtration 3750 Gel filtration 3755 elution 3760 elution 3761 elution 3762 antibodies 3762 antibodies against 3762 gel-filtration 3762 Immunoblots 3764 NMR 3764 spectroscopy 3765 NMR 3766 NMR 3766 proteolysis experiments 3767 NMR 3767 spectroscopy 3769 chemical shifts 3769 HSQC 3769 NMR 3770 ARIA/CNS protocol 3771 HSQC 3772 NMR 3772 titration 3772 titration experiments 3776 Chemical shift 3777 NMR 3777 spectroscopy 3778 Chemical shift 3779 NMR 3780 HSQC 3780 titration 3781 chemical shift 3782 chemical shift 3782 titration 3783 NMR 3783 titrations 3784 chemical shift 3784 titration 3786 NMR 3786 NMR experiments 3787 NMR 3788 NMR 3789 chemical shifts 3791 overlay screens 3795 docking 3795 peptide array 3795 SPOT peptide array 3798 bait 3798 fusion protein 3799 sequenced 3800 sequenced 3803 complementation analysis 3803 fluorescence 3803 in vivo 3805 affinity chromatography 3805 chromatography 3810 fingerprinting 3810 MALDI-TOF 3810 mass spectrometry 3810 MS/MS 3810 peptide mass fingerprinting 3814 electrophoresis 3814 gel electrophoresis 3814 immunoblotting 3814 immunoprecipitation 3814 mass spectrometry 3814 mass spectrometry analyses 3814 Tandem mass spectrometry 3814 Tandem mass spectrometry analyses 3815 Immunoblotting 3819 purification 3819 Purification 3820 electrophoresis 3820 gel electrophoresis 3820 sodium dodecyl sulfate-polyacrylamide 3821 binding assay 3821 in vitro 3821 in vitro binding assay 3822 SDS gels 3823 purification 3823 SDS-PAGE 3824 mass spectrometry 3826 gel filtration 3827 copurify 3828 antibody 3828 immunoblot 3828 immunoblots 3828 pull-down 3829 antibody 3829 immunoblot 3829 immunoblots 3829 pull-down 3832 antibodies 3832 fusion proteins 3832 GST 3832 GST fusion 3832 Western blot 3833 fusion proteins 3833 GST 3833 in vitro 3833 pull down 3833 pull down assay 3835 GST 3835 GST pull down 3835 GST pull down assays 3835 pull down 3835 pull down assays 3836 fusion proteins 3836 GST 3838 GST 3838 GST pull-down 3838 GST pull-down experiments 3838 pull-down 3838 pull-down experiments 3840 fusion protein 3840 GST 3842 autoradiography 3842 glutathione Sepharose 3842 glutathione-Sepharose 3842 GST 3842 GST fusions 3842 immobilized 3842 in vitro 3842 Sepharose 3842 two-hybrid 3842 two-hybrid system 3843 GST 3843 in vitro 3843 SDS-PAGE 3843 SDS-PAGE analysis 3844 in vitro 3847 fusion proteins 3847 immobilized 3853 in vitro 3854 in vitro 3856 immobilized 3859 Chromatography 3861 coprecipitated 3861 in vivo 3864 coprecipitation 3864 coprecipitation analysis 3864 pulled down 3865 pulled down 3873 chimera 3873 glutathione-Sepharose 3873 GST 3873 in vitro 3874 fusion protein 3874 GST 3874 GST fusion 3874 pull-down 3874 pull-down assays 3874 two-hybrid 3874 yeast two-hybrid 3875 GST 3875 GST pull-down 3875 GST pull-down experiments 3875 in vitro 3875 pull-down 3875 pull-down experiments 3875 two-hybrid 3875 two-hybrid system 3875 yeast two-hybrid 3875 yeast two-hybrid system 3878 GST 3878 pull down 3882 antibody 3882 anti-GFP 3882 anti-GFP antibody 3882 GST 3882 western blot 3883 IP experiments 3886 co-purified 3886 in vivo 3888 in vitro 3889 GST 3890 GST 3891 autoradiography 3891 fusion proteins 3891 SDS-PAGE 3892 in vitro-translated 3894 in vivo 3895 coprecipitated 3896 copurified 3897 in vivo 3899 immobilized 3902 immobilized 3903 immobilized 3904 GST 3904 GST pull-down 3904 GST pull-down assay 3904 pull-down 3904 pull-down assay 3906 glutathione-Sepharose 3906 pull down 3906 pull-down 3906 pull-down experiment 3906 pulled down 3907 fusion protein 3907 GST 3907 immobilized 3908 immobilized 3909 fusion protein 3909 GST 3909 immobilized 3910 fusion protein 3910 GST 3910 immobilized 3911 GST 3911 GST pull-down 3911 GST pull-down experiments 3911 pull-down 3911 pull-down experiments 3913 copurify 3914 GST 3914 pull-down 3914 pull-down experiments 3918 in vitro 3918 pulled down 3919 in vitro 3919 pulled down 3920 in vitro 3920 in vitro translated 3921 GST 3922 pulls down 3923 pulls down 3924 in vitro 3924 in vitro translated 3925 fusion protein 3925 in vitro 3925 in vitro translated 3925 pull down 3926 pulls down 3928 in vitro 3928 in vitro translated 3928 pull down 3929 pull down 3929 pull-down 3929 pull-down assay 3932 pull-down 3933 GST 3934 GST 3936 in vitro 3936 in vitro translated 3937 in vitro 3938 fusion protein 3938 GST 3939 GST 3941 fusion protein 3941 in vitro 3941 in vitro translated 3941 pull-down 3941 pull-down assays 3943 GST 3944 Sepharose 3944 Sepharose beads 3945 fusion protein 3945 In vitro 3946 fusion protein 3946 GST 3946 in vitro 3946 pulled down 3947 GST 3951 co-precipitated 3951 GST 3953 GST 3953 GST pull-down 3953 GST pull-down assay 3953 pull-down 3953 pull-down assay 3955 fusion protein 3955 glutathione sepharose beads 3955 In vitro 3955 In vitro translated 3956 GST 3956 GST fusion 3956 pull down 3957 GST 3957 GST pull-down 3957 GST pull-down assay 3957 pull-down 3957 pull-down assay 3958 GST 3958 GST pull-down 3958 GST pull-down assay 3958 pull-down 3958 pull-down assay 3958 two-hybrid 3963 fusion protein 3963 in vitro 3965 fusion protein 3965 In vitro 3966 biotinylated 3966 fusion proteins 3966 GST 3966 GST fusion 3966 GST pull-down 3966 GST pull-down assay 3966 in vitro 3966 in vitro translated 3966 pull-down 3966 pull-down assay 3967 immunoblotting 3967 pulldown 3967 Western immunoblotting 3968 antibody 3968 anti-FLAG 3968 anti-FLAG antibody 3968 Immunoblotting 3969 in vitro 3969 Sepharose 3970 GST 3971 fusion proteins 3971 pull-down 3971 pull-down assay 3973 fusion protein 3973 GST 3973 GST fusion 3973 immunoblot 3973 immunoblot analysis 3973 SDS-PAGE 3974 coprecipitates 3975 antibody 3975 electrophoresis 3975 gel electrophoresis 3975 SDS-PAGE 3975 sodium dodecyl sulfate-polyacrylamide 3978 fusion protein 3978 GST 3978 pulldown 3978 pulldown assays 3979 fusion protein 3980 pull-down 3980 pull-down assays 3981 coimmunoprecipitation 3981 coimmunoprecipitation experiments 3981 pull-down 3982 fusion proteins 3983 in vitro 3984 glutathione Sepharose 3984 GST-tagged 3984 Sepharose 3984 Western blotting 3985 fusion protein 3985 GST 3986 fusion protein 3986 In vitro 3986 In vitro assays 3989 pull-down 3989 pull-down assays 3993 GST 3994 pulled down 3995 GST 3995 GST pull-down 3995 GST pull-down assays 3995 in vitro 3995 pull-down 3995 pull-down assays 3996 GST 3996 in vitro 3996 pulled down 3997 pull down 3998 coprecipitated 3998 GST 3999 antibodies 3999 anti-GFP 3999 coprecipitate 3999 fusion proteins 3999 GFP 3999 GST 3999 GST fusion 3999 Immunoblotting 4000 in vitro 4002 autoradiography 4002 SDS-PAGE 4004 fusion proteins 4004 GST 4004 GST pull-down 4004 GST pull-down assay 4004 pull-down 4004 pull-down assay 4004 pulled down 4005 pulled down 4007 in vitro 4007 in vivo 4008 fusion proteins 4008 precipitate 4009 affinity purification 4009 purification 4011 binding assay 4011 GST 4011 in vitro 4011 in vitro binding assay 4012 GST 4012 in vitro 4012 in vitro pull-down assays 4012 pull-down 4012 pull-down assays 4013 GST 4013 GST pull-down 4013 GST pull-down assays 4013 pull-down 4013 pull-down assays 4013 two-hybrid 4013 yeast two-hybrid 4014 GST 4014 GST pulldown 4014 GST pulldown experiments 4014 in vitro 4014 pulldown 4014 pulldown experiments 4015 in vitro 4018 pulled-down 4018 Western blot 4018 Western blot analysis 4019 pull-down 4019 pull-down experiments 4020 fusion protein 4020 pull-down 4020 pull-down experiments 4021 affinity purification 4021 fusion protein 4021 purification 4021 tandem affinity purification 4021 TAP 4021 Western blot 4021 Western blot analysis 4022 immobilized 4024 fusion protein 4024 GST 4024 in vitro 4024 in vitro pulldown 4024 in vitro pulldown assay 4024 pulldown 4024 pulldown assay 4026 Sepharose 4028 co-purification 4028 fusion proteins 4028 GST 4028 GST fusion 4028 immobilized 4029 antibody 4031 affinity chromatography 4031 chromatography 4031 Co purification 4031 purification 4032 GST 4032 GST fusion 4033 antibody 4033 Western-blot 4033 Western-blot analysis 4035 in vitro 4035 in vitro pull-down assays 4035 pull-down 4035 pull-down assays 4036 in vitro 4037 precipitate 4038 fusion protein 4039 fusion protein 4039 in vitro 4040 Pull-down 4040 Pull-down assay 4041 in vitro 4042 co-purification 4042 co-purification experiments 4042 in vivo 4043 In vitro 4045 GST 4045 immobilized 4045 pull-down 4045 pull-down assay 4048 in vitro 4050 pull-down 4050 pull-down assay 4051 pulled down 4052 in vitro 4052 in vitro translated 4054 in vitro 4054 in vitro translated 4054 pull-down 4054 pull-down assays 4056 coimmunoprecipitation 4056 coimmunoprecipitation results 4056 in vivo 4056 pull-down 4063 affinity chromatography 4063 chromatography 4064 antibodies 4064 GST 4064 GST pulldown 4064 GST pulldown assays 4064 pulldown 4064 pulldown assays 4067 GST 4067 GST pulldown 4067 in vitro 4067 pulldown 4068 GST 4070 fusion protein 4070 glutathione Sepharose 4070 Sepharose 4070 Sepharose beads 4071 co-immunoprecipitation 4071 pulled down 4074 antibodies 4074 immunoblotting 4074 precipitates 4076 GST 4077 GST 4077 in vitro 4077 pulldowns 4078 GST 4080 in vitro 4081 GST 4081 GST fusion 4082 pulled-down 4086 fusion proteins 4086 GST 4086 GST fusion 4086 In vitro 4086 pull-down 4086 pull-down experiments 4087 fusion protein 4088 binding results 4088 In vitro 4088 In vitro binding results 4090 binding assays 4090 in vitro 4090 in vitro binding assays 4092 fusion protein 4092 GST 4092 GST fusion 4092 in vitro 4092 in vitro translated 4093 pulled down 4095 binding assays 4096 GST-tagged 4096 in vitro 4096 pull-down 4096 pull-down experiments 4096 pulled down 4096 Sepharose 4096 Sepharose beads 4097 binding assay 4097 GST 4097 pull down 4099 mass spectrometry 4099 mass spectrometry analysis 4100 GST 4100 GST pull-down 4100 GST pull-down experiments 4100 immobilized 4100 pull-down 4100 pull-down experiments 4101 Western blot 4101 Western blot analyses 4102 co-localizes 4103 fusion proteins 4103 in vitro 4103 in vitro translated 4103 pulled down 4103 Sepharose 4104 pull-down 4104 pull-down experiments 4105 in vitro 4107 fusion proteins 4107 pulled down 4107 Western blotting 4108 GST 4108 GST pull-down 4108 GST pull-down assay 4108 in vitro 4108 pull-down 4108 pull-down assay 4109 copurify 4111 copurify 4112 copurify 4115 biotinylated 4115 pulldown 4115 pulldown assay 4116 pulldown 4116 pulldown experiment 4119 Pulldown 4119 Pulldown assays 4120 Pulldown 4120 Pulldown experiments 4124 pull-down 4124 pull-down assays 4125 in vitro 4125 in vitro translated 4125 the Y2H screen 4126 co-precipitates 4127 co-precipitation 4127 co-precipitation assays 4127 GST 4129 fusion proteins 4130 affinity chromatography 4130 chromatography 4130 GST 4130 Immobilized 4130 in vitro 4130 two-hybrid 4131 GST-tagged 4131 pull-down 4131 pull-down assays 4132 antibody 4132 immunoblotting 4132 in vitro 4132 pull-down 4132 pull-down assays 4133 GST 4133 GST pull-down 4133 GST pull-down assays 4133 GST-tagged 4133 pull-down 4133 pull-down assays 4135 in vitro 4136 GST 4137 GST 4138 pulled down 4146 pull down 4146 pull down assay 4147 Pull down 4147 Pull down assay 4148 Pull down 4149 GST 4149 GST-tagged 4149 in vitro 4149 in vitro pulldown 4149 in vitro pulldown assays 4149 in vitro translated 4149 pulldown 4149 pulldown assays 4149 two-hybrid 4149 two-hybrid assays 4149 yeast two-hybrid 4149 yeast two-hybrid assays 4150 GST 4150 GST-tagged 4150 in vitro 4150 in vitro pulldown 4150 in vitro pulldown assays 4150 in vitro translated 4150 pulldown 4150 pulldown assays 4150 two-hybrid 4150 two-hybrid assays 4150 yeast two-hybrid 4150 yeast two-hybrid assays 4151 glutathione-Sepharose 4151 immobilized 4151 pull-down 4151 pull-down assay 4154 GST 4154 in vitro 4154 in vitro pulldown 4154 in vitro pulldown assay 4154 pulldown 4154 pulldown assay 4155 in vitro 4155 in vitro experiments 4156 GST 4156 in vitro 4159 antibody 4159 fusion proteins 4159 GST 4160 fusion proteins 4160 glutathione Sepharose 4160 immobilized 4160 Sepharose 4162 pull-down 4162 pull-down assays 4163 antibody 4163 immunoblot 4163 immunoblot analysis 4165 in vitro 4166 GST-pulldown 4166 GST-pulldown experiments 4167 chimera 4167 GFP 4167 in vivo 4167 Purification 4169 affinity matrix 4169 chimera 4169 GST 4169 GST pulldown 4169 GST pulldown assays 4169 in vitro 4169 in vivo 4169 pulldown 4169 pulldown assays 4171 GST 4171 GST pull-down 4171 GST pull-down assay 4171 pull-down 4171 pull-down assay 4177 GST 4177 GST pull-down 4177 GST pull-down experiments 4177 pull-down 4177 pull-down experiments 4179 in vitro-translated 4183 GST 4183 GST pull-down 4183 GST pull-down assay 4183 pull-down 4183 pull-down assay 4184 coexpression 4184 in vitro 4185 in vitro 4185 in vitro pull-down assays 4185 pull-down 4185 pull-down assays 4185 two-hybrid 4185 two-hybrid assays 4185 yeast two-hybrid 4185 yeast two-hybrid assays 4186 GST 4186 immobilized 4190 copurified 4191 purification 4193 precipitate 4199 fusion proteins 4199 GST 4199 GST fusion 4200 pull-down 4200 pull-down assays 4201 Pulldown 4201 Pulldown assay 4202 chromatography 4203 chromatography 4203 elution 4204 in vitro 4206 in vitro 4206 in vitro assays 4207 pull-down 4207 pull-down assays 4210 in vitro 4214 GST 4214 Sepharose 4214 Sepharose beads 4217 GST 4218 fusion protein 4218 reciprocal experiment 4220 antibody 4220 immunoblot 4220 immunoblot analysis 4220 pull-down 4220 pull-down analysis 4221 pull-down 4221 pull-down experiments 4223 coprecipitation 4223 glutathione-Sepharose 4223 GST 4224 fusion protein 4224 GST 4224 GST fusion 4224 in vitro 4224 pull down 4224 pull down experiment 4224 two hybrid 4224 two hybrid assay 4224 yeast two hybrid 4224 yeast two hybrid assay 4226 in vitro 4227 binding assays 4227 in vitro 4227 in vitro binding assays 4227 pull down 4228 In vitro 4228 In vitro pull-down assay 4228 pull-down 4228 pull-down assay 4229 in vitro 4229 in vitro pull-down assays 4229 pull-down 4229 pull-down assays 4229 two-hybrid 4229 two-hybrid assays 4232 anti-HA 4232 copurification 4232 pull down 4232 Western blotting 4233 fusion protein 4233 GST-pull-down 4233 GST-pull-down assay 4235 pull-down 4235 pull-down experiments 4236 immobilized 4239 GST-tagged 4239 in vitro 4239 in vitro translated 4239 pull-down 4239 pull-down assays 4241 Flag-tagged 4244 GFP 4244 green fluorescent protein 4244 GST 4244 GST pulldown 4244 GST pulldown assays 4244 pulldown 4244 pulldown assays 4245 FLAG-tagged 4245 GST 4245 GST pull-down 4245 GST pull-down experiment 4245 GST-tagged 4245 pull-down 4245 pull-down experiment 4246 GST-tagged 4249 glutathione-Sepharose 4249 GST 4249 immobilized 4249 pull-down 4249 pull-down assays 4250 affinity matrix 4250 fusion protein 4250 glutathione sepharose beads 4250 GST-tagged 4250 immobilized 4250 in-vitro-translated 4251 fusion proteins 4251 pull down 4253 in vitro 4253 in vitro assay 4254 pull-down 4254 pull-down assay 4255 GST 4255 GST pull-down 4255 GST pull-down assays 4255 pull-down 4255 pull-down assays 4257 pull-down 4258 GST-tagged 4258 pull-down 4258 pull-down assay 4260 GST 4260 GST pull-down 4260 GST pull-down assays 4260 pull-down 4260 pull-down assays 4262 pulled down 4263 GFP 4264 GST 4264 GST bound 4264 in vitro 4264 in vitro translated 4266 GST 4266 GST pull-down 4266 GST pull-down assays 4266 pull-down 4266 pull-down assays 4266 Western blot 4266 Western blot analysis 4270 chromatography 4270 decahistidine tag 4270 in vitro 4270 nickel chromatography 4271 GST-pulldowns 4272 GST 4272 GST pulldown 4272 GST pulldown assay 4272 pulldown 4272 pulldown assay 4273 GST 4273 GST bound 4273 in vitro 4275 pull-down 4275 pull-down assays 4276 affinity matrix 4276 GST 4277 in vitro 4277 Overlay experiments 4277 pull-down 4277 two-hybrid 4277 two-hybrid assays 4279 in vitro 4282 fusion protein 4282 GST 4282 GST fusion 4282 in vitro 4283 pull-down 4283 pull-down experiments 4284 copurified 4285 copurified 4285 purification 4286 GST 4286 GST pulldown 4286 GST pulldown method 4286 in vitro 4286 pulldown 4286 pulldown method 4287 antibody 4287 anti-FLAG 4287 anti-FLAG antibody 4287 immunoblotting 4288 in vitro 4289 antibodies 4289 antibodies against 4289 antibody 4289 GST 4289 immunoblotting 4289 SDS-PAGE 4290 fusion protein 4294 Western blot 4294 Western blot analysis 4297 in vitro 4298 affinity-purify 4298 biotinylated 4301 GST 4307 in vitro 4307 in vivo 4311 interaction assay 4311 in vitro 4313 co-precipitated 4314 fusion proteins 4314 GST 4314 GST fusion 4314 SDS gels 4316 glutathione-Sepharose 4316 GST 4316 Pulldown 4316 Pulldown assays 4319 polyacrylamide gel 4320 immunoblot 4320 immunoblot analysis 4320 Sepharose 4324 fusion proteins 4324 GST 4324 GST fusion 4324 pull-down 4324 pull-down assays 4328 Purification 4332 pull-down 4332 pull-down experiments 4334 in vitro 4334 in vivo 4335 pulled down 4336 antibody 4336 His tag 4336 purification 4336 Western blotting 4337 fusion protein 4337 GST 4337 GST fusion 4337 two-hybrid 4337 two-hybrid results 4337 yeast two-hybrid 4337 yeast two-hybrid results 4338 in vitro 4338 in vitro-translated 4340 HA-tagged 4340 in vitro 4340 transient transfection 4342 fusion proteins 4343 affinity column 4343 FLAG 4343 FLAG tag 4344 glutathione-Sepharose 4344 pulled down 4347 SDS-PAGE 4348 GST-pull-down 4348 GST-pull-down assays 4348 in vitro 4349 Autoradiography 4350 bait 4350 Pull-down 4351 in vitro 4352 in vitro 4353 GST 4353 GST pull-down 4353 GST pull-down assays 4353 immobilized 4353 pull-down 4353 pull-down assays 4354 in vitro 4357 pull down 4359 pulled down 4361 pulled down 4362 antibodies 4362 immunoblotting 4362 mass spectrometry 4363 GST 4363 GST pulldown 4363 GST pulldown assay 4363 pulldown 4363 pulldown assay 4364 crystal 4364 crystal structures 4364 GST 4364 GST pull-down 4364 GST pull-down assays 4364 HSQC 4364 NMR 4364 pull-down 4364 pull-down assays 4364 spectroscopy 4365 GST 4365 GST pull-down 4365 GST pull-down assays 4365 pull-down 4365 pull-down assays 4366 GST 4366 GST fusions 4366 pull-down 4366 pull-down assay 4366 pull-down assays 4367 fusion protein 4367 GST 4367 GST pulldown 4367 GST pulldown assays 4367 in vitro 4367 pulldown 4367 pulldown assays 4368 GST 4369 in vitro 4370 glutathione Sepharose 4370 GST 4370 GST fusion 4370 in vitro 4370 in vitro pull-down assays 4370 pull-down 4370 pull-down assays 4370 Sepharose 4370 Sepharose beads 4370 two-hybrid 4370 two-hybrid assay 4370 yeast two-hybrid 4370 yeast two-hybrid assay 4372 affinity matrix 4372 pulled down 4373 binding assay 4373 in vitro 4373 in vitro binding assay 4375 affinity matrix 4375 binding assays 4375 in vitro 4375 in vitro binding assays 4375 pulled down 4377 fusion protein 4377 in vitro 4381 docking 4381 GST 4381 in vitro 4382 co-precipitated 4382 Immunoprecipitation 4384 in vitro 4385 GST 4385 pulled down 4386 glutathione-Sepharose 4386 interaction analysis 4386 in vitro 4391 antibody 4391 Western blots 4395 co-precipitates 4396 GST 4397 Flag-tagged 4397 in vitro 4397 in vitro pull-down assay 4397 pull-down 4397 pull-down assay 4399 GST-bound 4401 GST 4401 GST pull-down 4401 GST pull-down assay 4401 pull-down 4401 pull-down assay 4401 two-hybrid 4401 two-hybrid analysis 4401 yeast two-hybrid 4401 yeast two-hybrid analysis 4403 GST-fused 4403 staining 4410 GST 4410 Pulldown 4412 in vitro-translated 4413 GST 4413 GST pull-down 4413 GST pull-down experiments 4413 pull-down 4413 pull-down experiments 4414 binding experiments 4414 fusion proteins 4414 in vitro 4414 in vitro binding experiments 4415 pull-down 4415 pull-down assay 4417 in vitro 4418 SDS-PAGE 4418 SDS-PAGE analysis 4419 co-precipitated 4420 in vitro 4420 in vitro translated 4421 co-precipitates 4422 bait 4422 pulled down 4423 antibody 4423 fusion protein 4423 pull down 4424 pulldown 4424 pulldown assay 4426 GST 4426 GST pull-down 4426 GST pull-down assays 4426 pull-down 4426 pull-down assays 4427 in vitro 4427 in vitro pulldown 4427 in vitro pulldown assay 4427 pulldown 4427 pulldown assay 4428 co-purified 4429 coprecipitated 4430 coprecipitated 4430 immunoblots 4430 precipitate 4430 precipitates 4430 pull-downs 4431 coprecipitated 4431 immunoblots 4431 precipitate 4431 precipitates 4431 pull-downs 4432 in vivo 4432 pull-down 4432 pull-down assays 4432 the Y2H system 4433 in vitro 4433 in vitro pulldown 4433 in vitro pulldown assay 4433 pulldown 4433 pulldown assay 4434 Coomassie blue staining 4434 mass spectrometry 4434 SDS-PAGE 4434 staining 4435 in vitro 4435 in vitro pulldown 4435 in vitro pulldown assay 4435 pulldown 4435 pulldown assay 4436 fusion protein 4436 GST 4436 GST fusion 4436 In vitro 4437 in vitro 4437 in vitro pull-down assay 4437 pull-down 4437 pull-down assay 4439 in vitro 4440 in vitro 4441 in vitro 4442 GST 4442 GST-tagged 4444 fusion protein 4444 pull down 4444 pull-down 4444 pull-down assays 4445 fusion protein 4445 GST 4446 fusion proteins 4446 GST 4446 pulled-down 4447 fusion proteins 4447 GST 4447 pulled-down 4450 coimmunoprecipitation 4450 Pull-down 4451 chromatography 4451 mass spectrometry 4451 SDS-PAGE 4451 sequenced 4453 antibody 4453 binding analysis 4453 chromatography 4453 immunoblot 4453 immunoblot analysis 4453 In vitro 4453 In vitro binding analysis 4461 GST 4463 chromatography 4465 GST 4466 two-hybrid 4466 two-hybrid assays 4466 yeast two-hybrid 4466 yeast two-hybrid assays 4467 fusion protein 4467 GST 4467 GST fusion 4467 in vitro 4467 in vitro translated 4468 Pulldown 4468 Pulldown experiments 4470 anti-FLAG 4470 immunoprecipitation 4470 pulldown 4471 GST 4471 HA-tagged 4471 immobilized 4471 pulled down 4472 GST 4473 immobilized 4474 anti-FLAG 4474 FLAG-tagged 4474 immobilized 4476 GST-fused 4476 in vitro 4477 GST 4477 GST fusion 4479 affinity chromatography 4479 antibody 4479 chromatography 4479 in vitro 4479 Western analysis 4484 GST 4484 immobilized 4485 mass spectrometry 4486 HA-tagged 4487 in vitro 4489 fusion protein 4489 GST 4489 GST fusion 4489 in vitro-translated 4491 co-immunoprecipitation 4491 fusion protein 4491 GFP 4491 GST 4491 GST fusion 4491 pull-down 4491 pull-down experiments 4492 co-immunoprecipitation 4492 co-immunoprecipitation experiments 4492 GST 4492 GST fusion 4492 pull-down 4492 pull-down experiments 4493 GST 4496 Immunoblotting 4496 pull down 4496 pull down assay 4497 GST 4498 pull down 4499 GST 4499 GST pull-down 4499 GST pull-down assays 4499 pull-down 4499 pull-down assays 4500 fusion protein 4500 in vitro 4502 mass spectrometry 4502 mass spectrometry analysis 4502 two-hybrid 4502 two-hybrid assay 4502 yeast two-hybrid 4502 yeast two-hybrid assay 4505 pulled-down 4505 SDS-PAGE 4505 staining 4506 in vitro 4506 pull-down 4509 GST 4514 GST 4514 GST bound 4514 in vitro 4514 In vitro 4514 In vitro pull-down assays 4514 in vitro translated 4514 pull-down 4514 pull-down assays 4515 GST 4517 antibody 4517 anti-FLAG 4517 anti-FLAG antibody 4517 fusion protein 4517 glutathione-Sepharose 4517 GST 4517 GST fusion 4517 immunoblot 4524 fusion proteins 4524 GST 4524 GST fusion 4527 GST 4527 GST pull-down 4527 GST pull-down assay 4527 pull-down 4527 pull-down assay 4528 GST 4528 GST pull-down 4528 GST pull-down assay 4528 in vitro 4528 pull-down 4528 pull-down assay 4528 SDS-PAGE 4528 Western blot 4529 fusion protein 4529 GST 4531 GST 4532 GST 4532 GST-fused 4532 immobilized 4532 pulled down 4532 pulled-down 4532 staining 4532 western blotting 4533 glutathione-Sepharose 4533 GST 4533 GST pull-down 4533 GST pull-down assay 4533 pull-down 4533 pull-down assay 4534 glutathione-Sepharose 4534 GST 4535 GST 4535 immobilized 4537 GST 4537 In vitro 4537 In vitro pull-down assay 4537 pull-down 4537 pull-down assay 4538 GST-fused 4538 pull-down 4540 in vitro 4542 affinity matrix 4542 GST-tagged 4542 pulldown 4542 pulldown assay 4543 affinity matrix 4543 pulldown 4543 pulldown assay 4544 Autoradiography 4544 immobilized 4544 in-vitro-translated 4547 antibody 4548 chromatography 4549 in-vitro-translated 4550 fusion protein 4551 GST-fusion 4552 FLAG-tagged 4552 GST-tagged 4553 Pull down 4554 antibody 4554 anti-HA 4555 immunoblots 4556 prey 4557 prey 4558 prey 4559 GST 4559 GST pulldown 4559 GST pulldown assays 4559 pulldown 4559 pulldown assays 4560 GST 4560 prey 4561 autoradiography 4562 copurified 4562 GST 4562 GST-fused 4563 bait 4563 prey 4564 GST 4564 GST pulldown 4564 GST pulldown assays 4564 pulldown 4564 pulldown assays 4566 in vitro 4566 in vitro pull-down assays 4566 pull-down 4566 pull-down assays 4569 pull down 4571 antibody 4571 monoclonal antibody 4573 glutathione Sepharose 4573 GST 4573 Pull-down 4573 Pull-down assays 4573 Sepharose 4574 immobilized 4576 in vitro 4576 in vivo 4578 in vitro 4582 affinity chromatography 4582 chromatography 4582 GST 4582 GST pulldown 4582 GST pulldown assay 4582 pulldown 4582 pulldown assay 4583 in vivo 4584 in vitro 4585 GST 4585 In vitro 4598 fusion protein 4598 in vitro 4598 in vitro translated 4600 in vitro 4601 GST-fusion 4601 in vitro 4602 GST-fusion 4602 in vitro 4604 pull-down 4604 pull-down assay 4605 co-purified 4606 in vitro 4607 in vitro 4607 in vitro translated 4608 GST 4610 antibodies 4610 glutathione-Sepharose 4610 GST-tagged 4610 staining 4611 GST 4612 in vitro-translated 4612 pull-down 4612 pull-down experiments 4614 antibodies 4614 pull-down 4614 Western-blot 4614 Western-blot analysis 4616 mass spectrometry 4616 mass spectrometry analysis 4616 pull-down 4616 pull-down assay 4618 in vitro 4620 GST 4620 pull-down 4622 immobilized 4624 antibody 4624 immunoblotting 4628 pulled down 4628 Sepharose 4628 Sepharose beads 4629 pulled down 4631 coprecipitated 4631 in vivo 4632 SDS-PAGE 4633 GST 4635 GST 4635 GST pulldown 4635 GST pulldown experiments 4635 pulldown 4635 pulldown experiments 4636 binding assay 4636 in vitro 4636 in vitro binding assay 4637 in vitro 4637 in vitro pull-down assay 4637 pull-down 4637 pull-down assay 4638 binding assay 4638 in vitro 4638 in vitro binding assay 4639 GST 4639 GST pull-down 4639 GST pull-down assay 4639 pull-down 4639 pull-down assay 4640 pulled down 4641 GST-pull-down 4641 GST-pull-down experiments 4642 GST 4642 GST-pull-down 4642 GST-pull-down experiments 4643 antibodies 4643 pull-down 4643 pull-down assay 4643 staining 4643 Western blot 4645 GST 4645 GST pull-down 4645 GST pull-down analysis 4645 pull-down 4645 pull-down analysis 4650 GST 4650 GST pulldown 4650 pulldown 4651 in vitro 4654 affinity chromatography 4654 chromatography 4654 immobilized 4654 MALDI-TOF 4654 mass spectrometry 4655 GST 4655 GST pull-down 4655 GST pull-down experiments 4655 pull-down 4655 pull-down experiments 4661 pulled-down 4664 pull-down 4664 pull-down assay 4665 in vitro 4665 in vitro translated 4665 pull-down-assay 4666 antibody 4666 fusion proteins 4666 GST 4666 GST fusion 4666 GST pull-down 4666 GST pull-down assays 4666 pull down 4666 pull-down 4666 pull-down assays 4666 western blot 4667 antibody 4667 fusion protein 4667 GST 4667 GST pull-down 4667 GST pull-down assay 4667 pull-down 4667 pull-down assay 4669 GST 4669 GST pull-down 4669 GST pull-down assay 4669 pull-down 4669 pull-down assay 4670 co-purified 4670 His6-tag 4672 in vitro 4674 in vitro 4675 GST 4675 GST pull-down 4675 GST pull-down assays 4675 pull-down 4675 pull-down assays 4675 pulled down 4677 GST 4677 Pull-down 4677 Pull-down assay 4678 GST 4678 in vitro-translated 4679 In vitro 4680 GST-pulldown 4680 GST-pulldown assays 4681 affinity column 4681 GST 4681 GST pulldown 4681 GST pulldown assay 4681 pulldown 4681 pulldown assay 4682 in vitro-translated 4683 fusion protein 4683 GST 4684 bait 4684 pull-down 4684 pull-down assays 4684 pulled down 4687 pull down 4687 pull down assays 4689 immunoblot 4689 pull-down 4694 GST 4696 pull-down 4696 pull-down assay 4698 elution 4698 GST 4698 polyacrylamide gel 4699 copurification 4699 co-purification 4699 in vitro 4700 copurification 4700 co-purification 4700 in vitro 4701 copurification 4701 co-purification 4701 in vitro 4702 copurification 4702 co-purification 4702 in vitro 4703 GST 4703 GST-fusion 4703 GST pulldown 4703 pulldown 4706 pull-down 4706 pull-down assays 4707 GST 4708 GST 4708 pull down 4708 pull-down 4709 antibody 4709 GST 4709 GST pull-down 4709 GST pull-down experiments 4709 immunoblotting 4709 pull-down 4709 pull-down experiments 4710 antibody 4710 GST 4710 GST pull down 4710 GST pull down experiments 4710 pull down 4710 pull down experiments 4710 western blotting 4711 antibody 4711 GST 4711 GST pull down 4711 GST pull down experiments 4711 pull down 4711 pull down experiments 4711 western blotting 4714 in vitro 4716 antibody 4719 biotinylated 4721 elution 4722 coprecipitation 4722 coprecipitation assay 4722 fusion protein 4723 antibody 4723 co-purified 4723 fusion protein 4723 GST 4723 GST fusion 4723 immobilized 4723 interaction assay 4723 in vitro 4724 Autoradiography 4724 pulled down 4727 two-hybrid 4727 two-hybrid screening 4727 yeast two-hybrid 4729 GST 4729 GST fused 4729 in vitro-translated 4729 pull down 4731 in vitro 4732 GST 4732 pulldown 4734 GST 4736 mass-spectrometry 4738 antibodies 4738 coimmunoprecipitation 4738 coimmunoprecipitation experiments 4738 in vivo 4739 GST 4739 interaction assays 4741 GST 4744 fusion protein 4747 Copurify 4749 Coomassie blue staining 4749 SDS PAGE 4749 staining 4750 Coomassie blue staining 4750 SDS-PAGE 4750 staining 4751 immobilized 4751 in vitro 4751 SDS-PAGE 4752 coprecipitated 4753 fusion protein 4753 GST 4754 fusion protein 4755 fusion protein 4758 fusion protein 4763 fusion protein 4763 GST 4764 fusion protein 4764 GST 4764 GST fusion 4767 antibody 4770 antibody 4770 co-precipitates 4770 glutathione-Sepharose 4770 GST 4770 Western blot 4770 Western blot analysis 4771 In vivo 4773 immobilized 4775 affinity column 4775 elution 4775 immunoblot 4775 immunoblot analysis 4778 In vitro 4779 immobilized 4780 antibody 4780 GST 4780 in vitro 4781 fusion protein 4781 pull-down 4782 immunoblotting 4782 SDS-PAGE 4782 staining 4784 in vitro 4785 copurify 4785 immobilized 4787 chromatography 4787 Purification 4788 coimmunoprecipitation 4788 coimmunoprecipitation experiments 4788 in vivo 4795 GST 4797 fusion protein 4797 pull down 4798 immunoblot 4800 immunoblot 4801 Purification 4803 co-immunoprecipitate 4804 antibody 4804 immunoblots 4804 monoclonal antibody 4805 immunoblot 4807 GST 4807 in vitro 4808 GST 4809 GST 4811 In vitro 4814 binding experiment 4814 GST 4814 in vitro 4815 fusion protein 4817 in vitro 4818 in vitro 4821 GST 4821 two-hybrid 4821 two-hybrid system 4821 Western analysis 4822 antibodies 4822 anti-HA 4822 Western analysis 4825 GST 4826 immunoblotting 4827 GST 4827 immobilized 4827 in vitro 4827 Sepharose 4828 binding assay 4828 In vitro 4828 In vitro binding assay 4829 fusion protein 4829 GST 4829 GST fusion 4837 in vitro 4838 affinity columns 4838 two-hybrid 4838 two-hybrid system 4840 fusion protein 4842 antibody 4842 anti-HA 4842 GST 4842 pull-down 4842 pull-down experiments 4843 GST 4843 GST pull-down 4843 interaction assay 4843 pull-down 4845 GST-tagged 4845 immunoblot 4845 immunoblot analysis 4848 in vitro 4848 in vitro pull-down experiment 4848 pull-down 4848 pull-down experiment 4850 GST 4850 GST pull-down 4850 GST pull-down assay 4850 pull-down 4850 pull-down assay 4851 GST-fusion 4851 in vitro 4851 protein-binding assays 4854 antibodies 4854 immunoblotting 4855 pull-down 4856 GST 4856 GST fusion 4857 GSH beads 4859 in vitro 4859 in vitro pull-down assay 4859 pull-down 4859 pull-down assay 4860 affinity chromatography 4860 affinity chromatography assays 4860 chromatography 4860 chromatography assays 4860 staining 4862 Western blot 4863 chromatography 4863 Western blot 4864 GST-tagged 4864 in vitro 4864 In vitro 4865 GST-tagged 4865 in vitro 4865 in vitro pull-down assays 4865 pull-down 4865 pull-down assays 4866 In vitro-translated 4866 pulled down 4867 GST 4868 GST-tagged 4870 pull-down 4870 pull-down assay 4873 electrophoresis 4873 gel electrophoresis 4873 Mass spectrometry 4873 trypsin digest 4874 GST 4874 GST pull-down 4874 GST pull-down assay 4874 in vitro 4874 in vitro translated 4874 pull-down 4874 pull-down assay 4875 GST 4875 immobilized 4876 GST 4876 GST pull-down 4876 GST pull-down analysis 4876 In vitro 4876 pull-down 4876 pull-down analysis 4877 binding assays 4879 GST 4882 GST 4882 in vitro 4882 pull down 4882 pulldowns 4883 in vitro 4883 pull down 4884 pull down 4884 pull down results 4884 pulled down 4885 glutathione-Sepharose 4885 GST 4886 fusion protein 4887 fusion protein 4893 pull-down 4894 pulldown 4894 pulldown assays 4894 pulled down 4897 pulled down 4898 pull-down 4898 pull-down assay 4899 pull down 4900 binding assay 4900 GST 4900 immobilized 4900 In vitro 4900 In vitro binding assay 4901 copurified 4901 purification 4901 purification experiments 4902 antibodies 4902 immunoblotting 4902 pull-down 4902 pull-down assays 4903 coimmunoprecipitate 4903 FLAG 4903 GST 4904 GST 4904 pull down 4905 GST 4905 pull down 4905 Sepharose 4905 Sepharose beads 4907 pull-down 4907 pull-down assays 4909 precipitate 4910 pull-down 4910 pull-down assay 4915 in vitro-translated 4915 pull-down 4915 pull-down assays 4919 pull-down 4919 pull-down assay 4920 fusion protein 4920 GST 4920 GST fusion 4922 GST-tagged 4924 GST-tagged 4926 bait 4926 prey 4927 In vitro 4927 In vitro translated 4928 binding assay 4928 FLAG-tagged 4928 immunopurified 4928 in vitro 4928 in vitro translated 4930 light-scattering 4931 light-scattering 4937 surface plasmon resonance 4938 ITC 4939 immobilized 4939 sensor chip 4940 SPR analysis 4943 SPR assay 4946 immobilized 4948 methyltransferase assay 4949 antibodies 4949 sensor chip 4949 SPR analysis 4950 immobilized 4950 Surface plasmon resonance 4950 Surface plasmon resonance analysis 4952 surface plasmon resonance 4955 fusion protein 4955 purification 4956 chip matrix 4956 immobilized 4957 immobilized 4958 chip matrix 4958 immobilized 4960 surface plasmon resonance 4961 equilibrium binding 4961 immobilized 4962 immobilized 4962 sensor chip 4963 immobilized 4964 surface plasmon resonance 4965 surface plasmon resonance 4966 surface plasmon resonance 4967 surface plasmon resonance 4968 Surface plasmon resonance 4968 Surface plasmon resonance analyses 4970 in vitro 4970 in vitro assay 4970 spectroscopy 4970 surface plasmon resonance 4972 immobilized 4974 surface plasmon resonance 4975 spectroscopy 4975 surface plasmon resonance 4978 in vitro 4981 surface plasmon resonance 4982 surface plasmon resonance 4983 immobilized 4987 bait 4987 prey 4987 prey plasmids 4991 bait 4991 prey 4998 two-hybrid 4998 two-hybrid assay 5011 bait 5013 bait 5013 prey 5014 bait 5014 prey 5015 fusion proteins 5020 fusion protein 5021 fusion protein 5021 Fusion proteins 5023 two-hybrid 5023 two-hybrid assays 5023 yeast two-hybrid 5023 yeast two-hybrid assays 5024 bait 5025 crystal 5025 crystallizes 5026 Crystal 5026 Crystal structure 5028 bait 5028 prey 5028 prey plasmids 5031 crystals 5032 crystal 5032 crystal structure 5037 crystal 5037 crystal structure 5038 crystal 5038 crystal structure 5039 Crystals 5040 in vitro 5041 Crystal 5041 Crystal structure 5042 crystal 5042 crystal structure 5043 x-ray crystallography 5045 Crystals 5045 molecular replacement 5046 molecular replacement 5047 crystal 5047 crystal structure 5048 crystal 5048 crystal structure 5049 crystal 5049 crystal structures 5050 crystal 5050 crystal structure 5050 molecular replacement 5051 crystal 5051 Crystals 5053 crystal 5053 Crystals 5053 crystal structure 5053 molecular replacement 5054 crystal 5055 crystals 5055 molecular replacement 5056 crystals 5057 crystal 5057 crystal structure 5058 crystal 5059 crystallize 5061 Crystal 5061 Crystal structure 5062 crystal 5062 crystal structure 5063 crystals 5065 crystal 5065 crystal structure 5066 crystal 5066 crystal structure 5067 crystal 5069 crystal 5069 crystal structure 5069 purification 5071 crystal 5071 crystal structures 5073 X-ray 5073 X-ray crystallography 5074 crystals 5076 crystal 5076 crystal structures 5077 crystal 5077 crystal structures 5078 crystal 5078 crystal structures 5079 Crystals 5080 crystal 5080 crystal structure 5080 X-ray 5082 crystal 5082 crystal structure 5083 crystal 5084 crystal 5088 crystal 5089 Crystals 5091 X-ray 5097 crystals 5102 crystal 5102 crystal structure 5103 crystals 5105 crystals 5108 fusion protein 5109 Western blotting 5111 antibody 5111 Western blot 5111 Western blot analysis 5112 chromatography 5113 chromatography 5116 Edman degradation 5120 Chromatography 5121 electrophoresis 5121 elution 5121 gel electrophoresis 5122 Two-hybrid 5123 Two-hybrid 5125 comigrated 5125 HA-tagged 5127 antibodies 5128 antibodies 5129 in vitro 5130 antibodies 5130 antibodies against 5130 Western analysis 5131 electrophoresis 5131 fractionation 5131 gel electrophoresis 5131 Western blot 5131 Western blot analysis 5134 antibodies 5134 antibodies against 5134 immunodetection 5138 SDS gel 5146 SDS gel 5147 SDS gel 5156 electrophoresis 5156 gel electrophoresis 5161 2D-gel 5161 2D-gel electrophoresis 5161 electrophoresis 5161 knock-down 5161 knockout 5168 electrophoresis 5168 gel electrophoresis 5169 immunoblot 5170 electrophoresis 5170 gel electrophoresis 5171 coexpression 5174 Coexpression 5175 Coexpression 5184 two-hybrid 5184 two-hybrid analysis 5184 yeast two-hybrid 5184 yeast two-hybrid analysis 5185 two-hybrid 5185 two-hybrid analysis 5185 yeast two-hybrid 5185 yeast two-hybrid analysis 5186 two-hybrid 5186 two-hybrid analysis 5186 yeast two-hybrid 5186 yeast two-hybrid analysis 5187 two-hybrid 5187 two-hybrid analysis 5187 yeast two-hybrid 5187 yeast two-hybrid analysis 5188 two-hybrid 5188 two-hybrid analysis 5188 yeast two-hybrid 5188 yeast two-hybrid analysis 5189 two-hybrid 5189 two-hybrid analysis 5189 Yeast two-hybrid 5189 Yeast two-hybrid analysis 5192 colocalizes 5194 immobilized 5195 coexpression 5202 polyacrylamide gel 5206 cDNA library 5206 hybridization 5206 sequenced 5207 ChIP 5207 ChIP assay 5208 antibodies 5210 depletion 5211 antibodies 5211 antibody 5211 siRNA 5213 antibodies 5213 ChIP 5213 chromatin immunoprecipitation 5213 immunoprecipitation 5214 ChIP 5214 ChIP analysis 5216 antibody 5216 anti-FLAG 5216 anti-FLAG antibody 5221 antibodies 5222 ChIP 5223 ChIP 5223 ChIP experiments 5223 knockdown 5224 ChIP 5224 ChIP experiments 5225 ChIP 5225 ChIP analysis 5225 Chromatin immunoprecipitation 5225 immunoprecipitation 5226 antibody 5226 ChIP 5226 Chromatin immunoprecipitations 5226 immunoprecipitations 5227 antibody 5227 anti-FLAG 5227 anti-FLAG antibody 5227 ChIP 5227 Chromatin immunoprecipitation 5227 immunoprecipitation 5227 PCR 5227 PCR experiments 5230 ChIP 5230 ChIP assays 5230 chromatin immunoprecipitation 5230 immunoprecipitation 5231 antibody 5231 immunoprecipitation 5232 coexpression 5234 electrophoresis 5234 gel electrophoresis 5235 ChIP 5235 ChIP assays 5235 chromatin immunoprecipitation 5235 immunoprecipitation 5236 ChIP 5236 ChIP assays 5236 chromatin immunoprecipitation 5236 immunoprecipitation 5237 ChIP 5237 ChIP analyses 5237 Chromatin immunoprecipitation 5237 colocalizes 5237 immunoprecipitation 5239 ChIP 5241 antibody 5241 immunoprecipitates 5252 In vitro 5253 In vitro 5263 Fluorescence 5267 electrophoresis 5269 competition binding 5270 Competitive binding 5271 Competitive binding 5272 immobilized 5274 binding analyses 5275 binding analysis 5275 biotinylated 5275 immobilized 5275 Saturation binding 5275 Saturation binding analysis 5276 Competition assays 5276 immunoblotting 5276 in vitro 5277 competition assays 5283 autoradiography 5284 in vitro 5287 binding assay 5288 binding results 5288 ELISA 5288 fusion proteins 5288 GST 5288 GST fusion 5289 binding assay 5292 binding assay 5293 ELISA 5294 in vitro 5296 ELISA 5299 binding assay 5300 ELISA 5300 ELISA assay 5302 ELISA 5305 ELISA 5305 ELISA assay 5306 binding assay 5306 enzyme-linked immunosorbent assay 5306 in vitro 5307 antibody 5307 anti-GFP 5307 anti-GFP antibody 5307 supershifts 5313 Electrophoretic mobility 5313 Electrophoretic mobility shift assay 5313 EMSA 5313 in vitro 5313 supershift 5314 antibody 5316 antibody 5316 EMSA 5317 antibody 5317 EMSA 5318 comigrated 5321 electrophoretic mobility 5321 electrophoretic mobility shift assays 5321 in vitro 5321 in vitro translated 5325 electrophoresis 5326 gel shift 5327 biotinylated 5329 gel mobility shift 5329 gel mobility shift assay 5330 gel shift 5331 EMSA 5331 EMSA experiments 5332 EMSA 5334 EMSA 5335 EMSA 5336 EMSA 5336 supershift 5336 supershift analysis 5338 Electrophoretic mobility 5341 binding assays 5341 in vitro 5343 depletion 5343 EMSA 5344 Gel-shift 5344 Gel-shift analysis 5345 EMSA 5346 DNA-protein interaction 5347 antibodies 5349 gel mobility shift 5349 gel mobility shift experiments 5354 ChIP 5355 EMSA 5357 electrophoretic mobility 5357 electrophoretic mobility shift assay 5364 in vitro 5366 gel mobility shift 5366 gel mobility shift assay 5370 EMSA 5372 EMSA 5372 EMSA analyses 5373 ChIP 5373 chromatin immunoprecipitation 5373 immunoprecipitation 5373 PCR 5373 siRNA 5374 ChIP 5374 ChIP analysis 5374 PCR 5375 EMSA 5376 EMSA 5379 antibody 5379 gel shift 5379 supershift 5380 EMSA 5381 in vitro 5382 in vitro 5383 in vitro 5384 SDS-PAGE 5387 in vitro 5390 in vitro 5391 in vitro 5396 fusion protein 5397 Proteolytic cleavage 5398 activity assays 5400 in vivo 5401 in vitro 5401 in vitro assay 5417 in vitro 5418 In vitro 5421 fusion proteins 5421 in vitro 5421 ubiquitination assays 5426 Coomassie blue staining 5426 SDS-PAGE 5426 staining 5427 chromatography 5427 immunoprecipitates 5432 in vitro 5438 in vitro 5440 co-localization 5441 co-localization 5441 staining 5445 Colocalization 5446 FRET 5447 antibody 5447 in vivo 5447 monoclonal antibody 5449 immunostaining 5449 immunostaining techniques 5451 antibody 5451 fusion protein 5451 microscopy 5451 staining 5452 colocalizes 5457 Colocalize 5458 immunofluorescence 5459 staining 5460 colocalizes 5461 localize 5462 Co-localize 5467 co-localizes 5468 Flag-tagged 5469 in vivo 5471 colocalize 5471 immunostaining 5472 colocalize 5472 immunostaining 5474 co-localize 5475 colocalization 5476 colocalize 5476 microscopy 5479 antibody 5479 staining 5483 colocalize 5485 staining 5488 in vivo 5488 localizes 5490 colocalization 5491 Co-localization 5492 antibody 5492 co localization 5492 co-localization 5492 fluorescence 5492 staining 5494 Colocalization 5496 fusion proteins 5497 colocalizes 5498 co-localize 5499 immunostaining 5499 staining 5501 Colocalization 5502 colocalizes 5502 fluorescence 5502 green fluorescence 5503 colocalization 5503 immunofluorescence 5503 Staining 5504 antibodies 5504 colocalization 5504 immunofluorescence 5504 indirect immunofluorescence 5508 co-localizes 5509 Co-localization 5510 co-localizes 5511 colocalizes 5513 colocalizes 5514 Co-localization 5515 Co-localization 5515 immunofluorescence 5515 indirect immunofluorescence 5516 co-localization 5516 double immunolabeling 5516 immunolabeling 5517 antibody 5517 immunolabeling 5521 co-localization 5523 colocalize 5524 colocalize 5525 colocalize 5526 colocalize 5527 colocalize 5528 colocalize 5535 Colocalization 5538 Immunofluorescence 5539 staining 5540 Immunofluorescence 5540 in situ 5542 colocalize 5542 Immunohistochemical 5544 Colocalisation 5545 co-localization 5546 colocalize 5546 GFP 5547 colocalizes 5548 colocalize 5550 antibodies 5550 antibodies against 5550 Immunocytochemistry 5550 Immunocytochemistry analyses 5553 Colocalisation 5554 microscopy 5556 Colocalisation 5563 co-localize 5564 co-localizes 5565 colocalizes 5566 colocalization 5566 colocalizes 5568 coexpression 5568 Colocalization 5568 immunofluorescence 5568 immunofluorescence analysis 5571 co-localization 5573 fluorescence 5578 Colocalization 5578 colocalize 5582 colocalizes 5582 localizes 5583 Immunofluorescence 5583 Immunofluorescence analysis 5585 immunostaining 5586 staining 5587 colocalization 5588 antibody 5590 colocalization 5592 Immunofluorescence 5592 Immunofluorescence microscopy 5592 microscopy 5593 colocalizes 5595 colocalization 5595 immunofluorescence 5595 immunofluorescence assay 5595 indirect immunofluorescence 5595 indirect immunofluorescence assay 5597 Immunofluorescence 5597 Immunofluorescence staining 5597 staining 5598 co-localization 5599 fluorescence 5600 Colocalization 5600 in vivo 5601 co-localizes 5602 colocalizes 5603 localizes 5604 Immunofluorescence 5605 localizes 5609 colocalize 5617 colocalization 5618 colocalizes 5619 colocalizes 5620 colocalizes 5621 Co-localization 5622 co-localization 5623 staining 5624 colocalization 5625 antibodies 5625 antibodies against 5625 colocalize 5625 in vivo 5626 colocalizes 5627 colocalizes 5628 colocalizes 5630 antibodies 5631 localize 5632 colocalization 5633 colocalizes 5634 colocalization 5634 colocalizes 5635 colocalization 5636 colocalizes 5637 colocalizes 5638 colocalization 5638 microscopy 5639 antibody 5639 Immunofluorescence 5639 Immunofluorescence analysis 5640 colocalization 5640 Immunofluorescence 5640 Immunofluorescence microscopy 5640 microscopy 5641 colocalization 5641 Immunofluorescence 5643 colocalization 5644 antibodies 5644 staining 5646 Colocalization 5650 localizes 5654 N-terminal sequencing 5654 N-terminal sequencing analysis 5655 MALDI-TOF 5655 mass spectrometry 5656 mass spectrometry 5656 N-terminal sequencing 5656 N-terminal sequencing results 5664 GST 5664 GST fusion 5674 radiolabel 5676 In vitro 5676 In vitro kinase assays 5676 kinase assays 5680 in vivo 5681 in vitro 5685 fusion proteins 5685 GST 5685 GST fusion 5685 kinase assay 5686 Immunopurified 5686 in vitro 5687 In vitro 5689 in vitro 5690 GST 5690 GST fusion 5691 in vitro 5691 phosphorylation assays 5693 fusion protein 5694 in vitro 5694 in vivo 5696 In vitro 5696 In vitro kinase assays 5696 kinase assays 5697 fusion protein 5697 GST 5697 GST fusion 5700 immunoprecipitation 5701 GST 5701 kinase assays 5702 GST 5704 in vitro 5705 in vitro 5709 In vitro 5709 phosphorylation experiment 5711 in vitro 5711 in vitro kinase analysis 5711 kinase analysis 5712 in vitro 5712 in vitro kinase assay 5712 kinase assay 5713 in vitro 5714 in vitro 5715 antibody 5715 anti-HA 5717 in vitro 5720 fusion proteins 5720 kinase assay 5724 in vitro 5727 autoradiography 5727 immunoprecipitate 5729 Kinase assays 5730 Kinase assays 5733 HA-tagged 5734 Kinase assays 5735 in vitro 5735 in vivo 5737 GST 5737 in vitro 5737 in vitro kinase assays 5737 kinase assays 5739 FLAG-tagged 5743 immunoprecipitates 5743 in vitro 5743 in vitro kinase assay 5743 kinase assay 5744 immunoprecipitates 5744 in vitro 5744 in vitro kinase assays 5744 kinase assays 5745 in vitro 5745 in vitro kinase assays 5745 kinase assays 5746 antibody 5746 in vitro 5746 in vitro kinase assay 5746 kinase assay 5749 Differential phosphorylation 5749 UV light 5752 cotransfection 5753 immunoprecipitates 5753 kinase assays 5754 antibody 5754 anti-HA 5754 HA-tagged 5754 In vitro 5754 kinase assays 5754 precipitates 5756 in vitro 5757 in vitro 5759 Western blotting 5761 in vitro 5762 interaction results 5762 Phosphorylation assays 5767 kinase assay 5770 kinase assay 5771 kinase assays 5772 in vitro 5772 pull-downs 5773 GFP-fused 5773 in vitro 5773 in vitro kinase assay 5773 kinase assay 5774 in vitro 5775 in vitro 5777 in vitro 5778 in vitro 5778 in vitro kinase assays 5778 kinase assays 5778 site-directed mutagenesis 5781 in vitro 5781 in vitro kinase assays 5781 kinase assays 5782 immunoprecipitates 5784 kinase assay 5788 GST 5789 GST 5789 in vitro 5790 GST 5790 in vitro 5790 kinase assay 5791 in vitro 5793 kinase assays 5795 HA-tagged 5795 in vitro 5803 in vitro 5803 in vivo 5804 in vitro 5804 in vitro kinase assay 5804 kinase assay 5809 GST 5809 in vitro 5810 In vitro 5813 Mass spectrometric analysis 5814 In vitro 5814 In vitro kinase assays 5814 kinase assays 5817 in vitro 5828 In vitro 5838 In vitro 5839 in vitro 5840 In vitro 5840 In vitro kinase assays 5840 kinase assays 5841 In vitro 5841 In vitro kinase assay 5841 kinase assay 5842 In vitro 5842 In vitro kinase assays 5842 kinase assays 5843 in vitro 5845 antibody 5845 in vitro 5845 SDS-PAGE 5845 western blot 5849 fusion protein 5849 immunoprecipitate 5852 GST 5854 antibodies 5854 in vitro 5859 in vitro 5861 In vitro 5861 In vitro kinase assays 5861 kinase assays 5863 in vitro 5863 phosphorylation assays 5864 in vitro 5865 in vitro 5866 in vitro 5869 immunoprecipitates 5870 in vitro 5871 confocal microscopy 5871 microscopy 5872 Immunostaining 5872 Immunostaining analysis 5872 knockdown 5872 localize 5872 RNAi 5875 fusion protein 5875 one-hybrid 5875 one-hybrid assays 5875 yeast one-hybrid 5875 yeast one-hybrid assays 5880 reporter gene 5883 in vitro 5890 in vitro 5897 GST 5897 in vitro 5897 western blot 5897 western blot analysis 5900 Coomassie staining 5900 HPLC 5900 N-terminal sequencing 5900 SDS-PAGE 5900 staining 5901 time course experiments 5902 gel staining 5902 HPLC 5902 staining 5916 binding experiments 5916 equilibrium binding 5916 equilibrium binding experiments 5919 methyltransferase assay 5923 Mutational analysis 5925 in vitro 5926 Fluorography 5926 Fluorography results 5927 Fluorography 5927 Fluorography results 5935 Chimera 5936 in vitro 5936 in vivo 5937 colocalization 5938 colocalizes 5939 staining 5941 colocalization 5941 fluorescence 5944 Cotransfection 5944 fluorescence 5945 colocalization 5946 co-localization 5947 co-localizes 5951 in vivo 5954 colocalization 5955 Co-localization 5955 confocal microscopy 5955 microscopy 5956 HA-tagged 5956 Immunofluorescence 5956 microscopy 5958 antibody 5958 fluorescence 5958 GFP 5958 staining 5959 antibodies 5961 in vitro 5969 colocalization 5970 confocal microscopy 5970 fusion proteins 5970 in vivo 5970 microscopy 5972 immunofluorescence 5972 immunofluorescence staining 5972 staining 5974 confocal microscopy 5974 fluorescence 5974 fusion proteins 5974 microscopy 5980 confocal microscopy 5980 microscopy 5981 immunofluorescence 5981 immunofluorescence microscopy 5981 microscopy 5984 confocal microscopy 5984 microscopy 5985 coexpression 5985 coexpression experiments 5985 colocalization 5987 colocalizes 5989 colocalization 5990 Colocalization 5993 colocalizes 5997 staining 5998 immunofluorescence 5999 Immunofluorescence 5999 Immunofluorescence microscopy 5999 microscopy 6001 colocalization 6001 fluorescence 6004 confocal microscopy 6004 in vivo 6004 microscopy 6005 fluorescence 6007 colocalization 6008 colocalizes 6008 in vivo 6009 antibody 6009 confocal microscopy 6009 microscopy 6009 monoclonal antibody 6010 coexpression 6012 co-localize 6013 co-localization 6014 colocalization 6016 Coexpression 6017 colocalization 6019 colocalize 6020 colocalization 6021 colocalization 6025 Co-localization 6027 colocalization 6032 Immunofluorescence 6032 Immunofluorescence microscopy 6032 Immunofluorescence microscopy analysis 6032 microscopy 6032 microscopy analysis 6033 Co-localization 6034 colocalization 6034 confocal microscopy 6034 microscopy 6035 cytoplasmic staining 6035 HA-tagged 6035 staining 6038 fluorescence 6038 GFP 6038 green fluorescence 6039 colocalization 6039 localize 6041 colocalization 6042 FLAG-tagged 6042 immunostaining 6042 immunostaining analysis 6047 colocalization 6047 GFP 6048 antibody 6048 staining 6049 antibody 6049 colocalize 6049 fusion protein 6049 staining 6055 fluorescence 6057 Co-localization 6058 colocalization 6058 nocodazole 6058 staining 6059 Confocal microscopy 6059 microscopy 6060 colocalize 6062 co-localization 6062 fluorescence 6062 green fluorescence 6064 colocalization 6064 labelling 6065 colocalization 6066 colocalization 6067 colocalization 6068 colocalization 6069 Co-localization 6070 colocalization 6070 immunofluorescence 6070 immunofluorescence analyses 6073 Co-localization 6074 Co-localization 6075 colocalization 6076 colocalization 6077 fusion protein 6078 colocalization 6078 confocal microscopy 6078 immunofluorescence 6078 immunofluorescence analysis 6078 in vivo 6078 localize 6078 microscopy 6079 colocalize 6082 Coexpression 6082 fluorescence 6082 FRET 6083 fluorescence 6083 in vivo 6084 colocalization 6085 colocalization 6086 co-localizes 6087 Colocalization 6092 antibodies 6093 co-localizes 6094 co-localization 6095 Localize 6100 confocal microscopy 6100 microscopy 6108 confocal microscopy 6108 microscopy 6110 colocalization 6111 colocalize 6111 in vivo 6112 colocalizes 6113 Colocalization 6114 colocalization 6115 colocalize 6119 Immunofluorescence 6119 Immunofluorescence analysis 6120 immunofluorescence 6121 Coexpression 6123 fusion proteins 6125 GFP 6127 fluorescence 6130 coexpression 6130 fluorescence 6131 Colocalization 6132 Coexpression 6133 coexpression 6133 coexpression experiments 6135 colocalization 6136 colocalizes 6137 antibodies 6137 staining 6140 Colocalization 6141 immunofluorescence 6141 immunofluorescence microscopy 6141 microscopy 6143 confocal microscopy 6143 confocal microscopy analysis 6143 immunofluorescence 6143 microscopy 6143 microscopy analysis 6145 confocal microscopy 6145 microscopy 6148 confocal microscopy 6148 microscopy 6149 Confocal microscopy 6149 microscopy 6151 siRNA 6156 colocalizes 6158 colocalizes 6159 affinity purification 6159 purification 6159 tandem affinity purification 6159 TAP 6163 in vivo 6163 purification 6164 mass spectrometry 6164 tandem mass spectrometry 6165 copurification 6165 Purification 6166 coisolation 6166 purification 6167 copurification 6167 purification 6168 copurification 6169 copurified 6169 MALDI-TOF 6169 mass spectrometry 6173 staining 6174 copurified 6174 mass spectrometry 6175 copurified 6175 mass spectrometry 6176 purification 6176 TAP 6177 copurified 6178 copurified 6178 Purification 6179 copurified 6179 purification 6182 copurified 6182 mass spectrometry 6183 affinity purifications 6183 purifications 6183 Tandem affinity purifications 6184 gel filtration 6184 mass spectrometry 6184 SDS-PAGE 6186 mass spectrometry 6186 mass spectrometry analysis 6186 MS/MS 6187 antibodies 6187 Immunoblotting 6188 elution 6189 TAP 6189 TAP screen 6190 purification 6190 TAP 6190 TAP results 6191 coprecipitated 6192 coprecipitated 6193 coprecipitated 6194 mass spectrometry 6194 pull-down 6196 mass spectrometry 6197 coprecipitated 6199 electrospray 6199 electrospray ionization 6204 TAP 6204 Western blot 6204 Western blot analysis 6205 TAP 6206 affinity purification 6206 purification 6206 tandem affinity purification 6207 copurify 6209 immunoprecipitates 6210 immunoprecipitates 6212 purification 6214 baits 6214 Copurification 6214 Western blot 6214 Western blotting 6217 co-purified 6217 pull-down 6217 pull-down experiments 6218 mass spectrometry 6218 purification 6218 tandem mass spectrometry 6219 pull-down 6221 MALDI 6221 MS/MS 6221 MS/MS analyses 6226 Mass spectrometry 6226 Mass spectrometry analysis 6230 antibody 6231 RPA 6232 baits 6232 cDNA library 6232 sequenced 6234 two-hybrid 6234 two-hybrid screen 6235 mammalian two-hybrid 6235 mammalian two-hybrid assay 6235 two-hybrid 6235 two-hybrid assay 6236 fusion protein 6241 electrophoresis 6241 gel electrophoresis 6241 Two-dimensional gel electrophoresis 6242 SDS-PAGE 6243 antibodies 6243 electrophoresis 6243 gel electrophoresis 6244 fluorescence 6245 fluorescence 6246 fluorescence 6247 fluorescence 6249 in vivo 6250 two-hybrid 6250 two-hybrid assay 6250 yeast two-hybrid 6250 yeast two-hybrid assay 6251 fluorescence 6254 ESR 6254 fluorescence 6254 fusion protein 6254 GFP 6254 green fluorescence 6254 green fluorescent protein 6255 ESR 6255 fluorescence 6255 GFP 6256 in vivo 6256 two-hybrid 6256 two-hybrid screen 6258 fluorescence 6258 localize 6260 fluorescence 6260 green fluorescence 6261 Complementation system 6261 fluorescence 6261 Fluorescence 6261 in vivo 6264 fluorescence 6265 fluorescence 6269 co-localization 6271 fluorescence 6272 complementation assay 6275 staining 6277 fluorescence 6278 fluorescence 6278 in vivo 6279 In vivo 6280 microscopy 6281 microscopy 6283 Flow cytometry 6284 FRET 6285 X-ray 6289 western blot 6289 western blot analysis 6292 chromatography 6294 In vitro 6298 Autoradiography 6298 GST 6299 prey